烟草野火病菌Pseudomonas syringae pv.tabaci yuexi-1信号肽预测及分析  

Analysis of coding region for proteins containing signal peptides of Pseudomonas syringae pv. tabaci yuexi-1 strain

作　　者：王铁霖[1] 李晶[1] 杨玉文[1] 赵廷昌[1] 

机构地区：[1]中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京100193

出　　处：《中国烟草学报》2016年第1期92-100,共9页Acta Tabacaria Sinica

基　　金：烟草病虫害检测与综合治理重点开放实验室开放课题经费(项目号:bc2011);中国烟草总公司科技重点项目(项目号110201202002)

摘　　要：利用Signal P 4.0、Lipo P 1.0及TMHMM v2.0对烟草野火病菌Pseudomonas syringae pv.tabaci yuexi-1菌株基因组中信号肽的数量、长度和氨基酸组成进行了预测及分类。结果确定其中432个ORFs（Open reading frame）所编码的N端有信号肽序列,占全部ORFs的8.81%。其中351条分泌型信号肽（SPI）,81条脂蛋白型信号肽（SPII）。在分泌型信号肽中,信号肽的长度为11~42个氨基酸,以长度为22个氨基酸的信号肽最多。同源性分析结果显示,具有相同信号肽序列的不同蛋白序列之间是高度保守的。该研究提供了野火病原菌致病因子的备选基因,提高该病菌致病因子的筛选效率。This paper predicted and analyzed the number, length and amino acid components in the genome of Pseudomonas syringae pv. tabaci yuexi-1, a strain of tobacco fire blight pathogen, by using Signal P 4.0, Lipo P 1.0 and TMHMM v2.0. Results showed that 432 ORFs（8.81% of all the ORFs） contained N- terminal signal peptides, of which 351 signal peptides were SPI type and 81 signal peptides were SPII type. Among the SPI peptides, length was between 11 to 42 amino acids and the majority was 22 amino acids in length. In addition, the proteins that share the same signal peptide sequences were highly conserved in the homologue analysis. This study provided candidate genes for virulence factors of the tobacco fire blight pathogen, and promoted the efficiency for screening the virulence factors.

关 键 词：Pseudomonas SYRINGAE pv.tabaci 信号肽 Signal P 4.0 Lipo P 1.0 TMHMM v2.0 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]