类风湿关节炎患者关节滑膜滑液中结缔组织生长因子表达及其意义研究  

Expression of CTGF in the synovial fluid and tissues of patients with rheumatoid arthritis and its significance

作　　者：丁爽[1] 吴春玲[1] 刘海娜[1] 肖卫国[1] 

机构地区：[1]中国医科大学附属第一医院风湿免疫科,辽宁沈阳110001

出　　处：《中国实用内科杂志》2016年第3期235-237,共3页Chinese Journal of Practical Internal Medicine

基　　金：辽宁省医学高峰建设工程专项经费项目任务实施方案(2010006)

摘　　要：目的检测结缔组织生长因子(connectivetissue growth factor,CTGF)在类风湿关节炎(rheumatoid arthritis,RA)患者关节滑液及滑膜中的表达,探索CTGF在RA关节病变中可能发挥的作用。方法采用酶联免疫法(enzyme-linked imm-unosorbent assay,ELISA)方法检测中国医科大学附属第一医院2013年1月至12月收治的10例RA患者和8例骨关节炎(osteoar thritis,OA)患者关节滑液CTGF水平;免疫组化法检测3例RA患者和2例OA患者关节滑膜CTGF的表达;Real-time PCR检测向体外培养的成纤维样滑膜细胞(FLS)中加入外源性肿瘤坏死因子α(tumor necrosis factor alpha,TNF-α)后对CTGF表达的影响。结果 RA组的关节滑液CTGF水平高于OA组[(21.00±10.20)μg/L对(8.53±7.71)μg/L)],差异有统计学意义(P<0.05);CTGF在RA滑膜组织主要表达于滑膜衬里细胞及衬里下层细胞,而OA滑膜组织仅少量CTGF表达;TNF-α可以上调FLS表达CTGF。结论 RA患者滑液CTGF水平升高,滑膜组织CTGF高表达,提示CTGF可能参与了RA关节炎症的发生发展。TNF-α上调FLS的CTGF表达,这可能是导致RA关节病变的重要因素之一。Objective To detect the expression of CTGF in the synovial fluid and tissues of patients with rheumatoid arthritis, explore the potential role of CTGF in rheumatoid arthritis synovial lesions. Methods Enzyme-linked immunosorbent assay（ELISA） was used to detect the CTGF levels of synovial fluid in 10 RA patients and 8 OA patients. Immunohistochemical detection was used to observe the CTGF expression in the synovial tissue of 3 RA patients and 2 OA patients. CTGF expression was detected by real-time PCR after TNF-α was added to FLS. Results CTGF in synovial fluid of RA patients was higher than that of OA patients（P0.05）. CTGF was mainly expressed in the synovial lining cells and sublining cells in RA synovial tissue, while only a small amount of CTGF expression in OA synovial tissue. The expression of CTGF in FLS could be up-regulated by TNF-α. Conclusion The increased CTGF level in synovial fluid and synovial tissues of RA indicates that CTGF may be involved in the development of RA. TNF-α can up-regulate the expression of CTGF in FLS, which may be one of the important factors that lead to RA joint disease.

关 键 词：结缔组织生长因子 类风湿关节炎 

分 类 号：R593.22[医药卫生—内科学]