实时荧光定量PCR法研究不同年龄段健康人群肠道菌群特点  

Characterization of fecal microbiota across different age groups by real-time fluorescence quantitative polymerase chain reaction

作　　者：刘萍[1] 郭珍红 董丽娜[2] 韩轶[3] 杨云峰[3] 王俊平[3] 

机构地区：[1]山西医科大学附属人民医院山西省人民医院妇产科,太原030012 [2]山西医科大学附属人民医院山西省人民医院中心实验室,太原030012 [3]山西医科大学附属人民医院山西省人民医院消化科,太原030012 [4]长治市第二人民医院消化科

出　　处：《中国临床实用医学》2016年第1期24-27,共4页China Clinical Practical Medicine

基　　金：山西省国际科技合作项目(2013081066)；山西省卫生厅攻关科研项目(201201059)Fund program:The International Science and Technology Cooperation Project of Shanxi，Science Foundation of Health and family planning commission of Shanxi Province

摘　　要：目的使用荧光定量PCR法分析新生儿至老年人不同年龄健康人群肠道菌群特点，比较各年龄段间的差异，探讨年龄对肠道菌群变化的影响。方法选择2014年11月至2015年2月山西省人民医院产科的新生儿及健康志愿者259名，分为胎儿组45名、婴幼儿组61名、少年组54名、成年组48名、老年组51名，提取各组人群新鲜粪便标本DNA，荧光定量PCR检测肠道双歧杆菌、乳酸菌、肠杆菌、类杆菌、肠球菌及梭菌细菌拷贝数、计算B／E值（双歧杆菌／肠杆菌），统计分析各组间差异。结果胎儿组和婴幼儿组双歧杆菌、肠杆菌、乳酸菌、类杆菌、肠球菌及梭菌数量间差异无统计学意义（P〉0．05）。与胎儿和婴幼儿组相比，老年组、成年组和少年组双歧杆菌、乳酸菌数量明显降低（P〈0．05），肠杆菌、肠球菌、类杆菌数量均明显升高（P〈0．05）。老年组和成年组双歧杆菌、乳酸菌数量亦明显低于少年组（P〈0．05）。老年组肠杆菌、肠球菌、类杆菌数量亦明显高于少年组和成年组（P〈0．05）；少年组和成年组肠杆菌、类杆菌、肠球菌数量组间差异无统计学意义（P〉0．05）。老年组、成年组和少年组B／E值明显低于胎儿组和婴幼儿组，老年组和成年组B／E值亦明显低于少年组，老年组B／E值亦明显低于成年组（P〈0．05）。结论年龄是肠道菌群变化的一个重要因素，随着年龄增长，肠道有益菌数量明显减少，而肠道中性菌数量明显增加，肠道定植抗力明显下降。Objective To analyze the characteristics of intestinal mierobiota during different age groups by quantitative PCR method, and to compare the differences among the age groups. Methods Collected the fetal meconium and fresh feces of infant group,youth group, adult group and elderly group,and preserved the samples regularly and strictly.6 major bacterial groups include Bifidobaeterium,Laetobaeillus, Enterobacteria,Bacterooides,Enterococcus and Clostridium were detected.Got the various intestinal bacterial copy number and B/E value of each samples by quantitative PCR technology.Used SPSS19.0 statistical software to process and analyze the data,and reviewed and analyzed the composition and characteristics of intestinal bacterial in different age. Results Quantitative PCR assay showed：The numbers of Bifibacterium, nterobacteriaeeae,Lactobacillus,Bacteroides,E, coli between fetuses group and infants group were not clear statistically insignificant（P〉0.05）.The numbers of Bifidobacterium and Lactobacillus in elderly group,adult group and juvenile group were obviously lower than those in fetuses and infants groups（P〈O.05）,while The numbers of Enterohaeteriaceae,Baeteroides in elderly group,adult group and juvenile group were obviously higher than those in fetuses and infants groups（P〈0.05）.The numbers of Bifidobacterium and Laetobaeillus in elderly group,aduh group were obviously lower than those in juvenile group（P〈0.05）,The numbers of Enterobacteriaeeae,E. Coli, Bacteroides in elderly group were obviously higher than those in adult group and juvenile groups （P〈0.05）.The numbers of Enterobacteriaceae,Bacteroides and E. coli between adult group and juvenile group were no statistically insignificant（P〉0.05）.The B/E values of Elderly,adult and juvenile group were obviously lower than that of fetuses and infants groups,the B/E values of Elderly and adults group were obviously lower than that of juvenile group,the B/E values of Elderly group were obviously lower than that of adults group,t

关 键 词：不同年龄阶段 健康人群 肠道微生态 16s RDNA PCR 

分 类 号：R446[医药卫生—诊断学]