膀胱过度活动症与尿液中神经生长因子相关性的临床研究  

Study on correlation between overactive bladder and nerve growth factor in urine

作　　者：侯瑞鹏[1] 李健[1] 李昭夷[1] 朱广博[2] 张蓓[2] 

机构地区：[1]天津市人民医院泌尿外科,天津300121 [2]天津市人民医院检验科

出　　处：《临床泌尿外科杂志》2016年第2期134-137,共4页Journal of Clinical Urology

基　　金：天津市卫生局科技基金(编号2013KZ060)

摘　　要：目的：探讨膀胱过度活动症（OAB）患者尿液中神经生长因子（NGF）的表达及与OAB严重程度的相关性,了解NGF能否作为OAB诊断的生物学标记物。方法：选择2012年5月~2015年3月我院收治的61例女性OAB患者（A组）,同期58例无OAB相关症状且OAB症状评分表（OABSS）正常的女性健康查体者作为正常对照组（B组）。两组患者均用酶联免疫吸附法（ELISA）检测尿液中NGF含量。A组给予口服索利那新治疗,持续用药4周后再次填写OABSS症状评分表、检测尿液中NGF含量。比较A、B两组尿液中NGF含量、OABSS症状评分差异及A组用药前后尿液中NGF含量、OABSS症状评分差异。每个检测样本尿液中NGF含量值除以该样本尿肌酐值（Cr）进行标准化。结果：A组与B组NGF/Cr分别为（1.682±0.988）和（0.737±0.356）,OABSS分别为（8.721±2.430）和（0.483±0.682）,二者比较差异有统计学意义（P〈0.01）。A组治疗前后尿液中NGF/Cr分别为（1.682±0.988）和（0.861±0.396）,OABSS评分分别为（8.721±2.430）和（3.197±1.470）,治疗前后比较差异有统计学意义（P〈0.01）。A组尿液中NGF/Cr与OAB严重程度呈显著的相关关系（r=0.704,P〈0.01）。结论：女性OAB患者尿液中NGF含量与OABSS评分同时升高,并且NGF的表达与OAB严重程度显著相关。经过索利那新治疗后OAB患者随着症状好转NGF明显降低。因此,NGF可作为OAB诊断的生物学标记物并用来判断疗效。Objective： To discuss the correlation between nerve growth factor （NGF） in urine of overactive bladder （OAB） patients and the severity of OAB and to examine whether urine NGF could serve as a hiomarker for the diagnosis of OAB. Method： From May 2012 to March 2015, 61 female OAB patients were involved in this re- search （A group）, while other 58 females without OAB symptoms and with normal OAB symptom score （OAB- SS） were selected as control group （B group）. The enzyme-linked immunosorbent assay （ELISA） was used to detect the content of NGF in the urine. Solifenacin was given to A group for four weeks. Then the OABSS was filled and the NGF in urine was tested again. The clinical data of the NGF in urine and OABSS between two groups were compared. Also, the clinical data of A group before and after medication were compared. The NGF value in each urine sample was divided by the urine creatinine （Cr） of sample for standardization. Result： The NGF/Cr of A and B group were 1. 682：J：0. 988 and 0. 737±0. 356 respectively and the OABSS of A and B group were 8. 721：[：2. 430 and 0. 483 ± 0. 682 respectively. The difference was statistically significant between groups （ P 〈20. 01）. The NGF/Cr in the urine of A group before and after treatment were 1. 682＋0. 988 and 0. 861±0. 396 respectively and the OABSS of A group before and after treatment were 8. 721±2. 430 and 3.197 q-1. 470 respectively. The differenee was statistieally significant （ P 〈0.01）. The NGF/Cr in urine of A group was significantly correlated with the severity of OAB （ r =0. 704, P d0.01）. Conclusion： The NGF in the urine and the OABSS of female OAB patients increased simultaneously and the NGF was relevant to the severity of OAB. Then the NGF of OAB pa- tients significantly reduced accompanied with the symptom remission after treatment of solifenacin. Hence, the NGF could be treated as a biomarker and efficacy judgment of OAB.

关 键 词：膀胱过度活动症 神经生长因子 生物学标记物 

分 类 号：R694.5[医药卫生—泌尿科学]