缺氧预处理对颅脑损伤大鼠皮质内质网应激蛋白P-eIF2α表达的影响  

Effect of hypoxic preconditioning on ERS protein, phosphorylated eIF2α,expression in rats after traumatic brain injury

作　　者：刘光杰[1] 刘家传[1] 王金标[1] 杨艳艳[1] 张星[1] 王春琳[1] 汤宏[1] 徐燊[1] 

机构地区：[1]安徽医科大学解放军临床学院解放军105医院神经外科,合肥230031

出　　处：《中国微侵袭神经外科杂志》2016年第2期83-86,共4页Chinese Journal of Minimally Invasive Neurosurgery

基　　金：全军医学科技"十二五"科研项目(编号:CWS11J262);南京军区联勤部卫生部"十一五"重点基金项目(编号:06Z19)

摘　　要：目的探讨缺氧预处理(hypoxic preconditioning,HPC)对颅脑损伤大鼠皮质内质网应激蛋白P-e IF2α表达的影响。方法将SD大鼠,随机分为对照组(n=8)、HPC组(n=8)、外伤组(TBI,n=96)、HPC+TBI组(HPCT,n=96)。采用改良Feeney’s法制作颅脑损伤大鼠模型,提前在低压氧舱内对大鼠处理3 d(-50 k Pa、3 h/d)。免疫组化法和Western blotting法检测挫伤区周围皮质P-e IF2α表达变化,TUNEL法检测凋亡神经元细胞。结果 P-e IF2α蛋白于颅脑损伤后3 h开始增加,6~12 h逐渐升高,1 d达高峰,3~14 d逐渐恢复;HPCT组与TBI组比较,伤后6 h^7 d P-e IF2α蛋白表达显著降低,差异有统计学意义(P<0.05)。TBI组、HPCT组伤后6 h可见凋亡细胞表达,12 h^1 d上升,3 d达高峰,7~14 d逐渐恢复;HPCT组与TBI组比较,伤后12 h^7 d凋亡率明显下降,差异有统计学意义(P<0.05)。结论 HPC可选择性抑制颅脑损伤大鼠皮质P-e IF2α蛋白表达,减轻内质网应激损伤,降低细胞凋亡率,维持神经元细胞功能稳定。Objective To explore the effect of hypoxic preconditioning on endoplasmic reticulum stress（ERS） protein, phosphorylated eukaryotic translation initiation factor 2α（P-eIF2α）, expression in traumatic brain injury rats. Methods Sprague Dawley rats were randomly divided into control group（n = 8）, hypoxic preconditioning group（HPC, n = 8）, traumatic brain injury group（TBI, n = 96） and traumatic brain injury after hypoxic preconditioning group（HPCT, n = 96）.The TBI model of rats were made by modified Feeney＇s method and hypoxic preconditioning was performed in a hypobaric chamber for 3 days（-50 k Pa, 3 h/d） in advance. Immunohistochemistry and Western blotting were used to detect the expression of P-e IF2α. The apoptosis of neurons was detected by TUNEL staining. Results The P-e IF2α protein appeared in 3 h after traumatic brain injury, and the appearance trended to increase from 6 to 12 h, then achieved its apex in 1 d and the trend decreased from 3 d to 14 d. The difference of appearance in 6 h-7 d was significant between the groups of HPCT and TBI（P〈0.05）. Apoptosis cells were detected by TUNEL staining, which appeared in 6 h after TBI, then trended to increase from 12 h to 1 d, achieved its apex in 3 d and the trend decreased from 7 d to 14 d. However, in the HPCT group, the apoptosis rate declined obviously in 12 h-7 d after TBI compared with TBI group, and the difference between the two groups was significant（P〈0.05）.Conclusions Hypoxic preconditioning can selectively inhibit the expression of P-e IF2α protein in the cortex of rats after TBI, reduce the ERS-related injury, decrease the rate of cell apoptosis, maintain the stability of neuron function.

关 键 词：颅脑损伤 缺氧预处理 内质网应激 大鼠 

分 类 号：R651.15[医药卫生—外科学]