高压干燥保存移植心脏机制的实验研究  

Experimental Research of the Mechanism of Preservation for Cardiac Transplantation in Dry Environment With High Air Pressure

作　　者：张瑞[1,2] 黄成锋[2] 李潇[2] 郑少忆[2] 郭惠明[2] 陈寄梅[2] 庄建[2] 朱平[1,2] 

机构地区：[1]南方医科大学,广东省广州市510515 [2]广东省人民医院广东省心血管研究所广东省医学科学院心外科

出　　处：《中国全科医学》2016年第9期1107-1112,共6页Chinese General Practice

基　　金：国家科技部国际合作项目(2010DFA32660)--一种全新的器官保存方法(心脏干燥保存)的实验研究;国家自然科学基金资助项目(81370230)--PGC-1α与SDF-1/CXCR4轴调控骨髓间充质干细胞促心肌梗死中血管新生的机制研究

摘　　要：目的探索一种全新的器官保存方式——高压干燥保存,并通过检测炎性因子水平探讨该方式优于目前常用的浸泡保存方式的可能作用机制。方法 2014年12月—2015年8月选取SPF级C57BL/6小鼠60只,其中4~6周龄供体小鼠36只,6~8周龄受体小鼠24只。供体小鼠采用随机数字表法分为空白对照组、组氨酸-色氨酸-酮戊二酸盐液（HTK液）浸泡保存组、高压干燥保存组,每组12只。受体小鼠采用随机数字表法分为HTK液浸泡保存受体组、高压干燥保存受体组,每组12只。空白对照组仅取供心,不做移植;HTK液浸泡保存组供心浸泡于HTK液中;高压干燥保存组供心悬挂并置于高压气体罐中（P_（O_2）：1 600 h Pa＋P_（CO）：400 h Pa=2 000 h Pa）。移植后2 h,对比观察HTK液浸泡保存受体组、高压干燥保存受体组供心复跳率及供心移植物功能评分。移植后24 h,采用荧光实时定量-聚合酶链式反应（RT-PCR）法检测3组供体小鼠心肌细胞肿瘤坏死因子α（TNF-α）、白介素（IL）1β、IL-6、IL-10、细胞间黏附分子1（ICAM-1）表达水平及心肌细胞凋亡指数。结果高压干燥保存受体组小鼠供心复跳率高于HTK液浸泡保存受体组〔83.3%（10/12）与25.0%（3/12）,P=0.012〕。高压干燥保存受体组小鼠供心移植物功能评分高于HTK液浸泡保存受体组〔3.5（1.0）分与1.3（0.5）分,Z=-3.447,P〈0.01〕。HTK液浸泡保存组和高压干燥保存组小鼠心肌细胞TNF-α、IL-1β、IL-6、IL-10、ICAM-1表达水平高于空白对照组（P〈0.05）;HTK液浸泡保存组小鼠心肌细胞TNF-α、IL-1β、IL-6、ICAM-1表达水平高于高压干燥保存组（P〈0.05）;高压干燥保存组小鼠心肌细胞IL-10表达水平高于HTK液浸泡保存组（P〈0.05）。空白对照组、HTK液浸泡保存组、高压干燥保存组小鼠心肌细胞凋亡指数分别为（1.36±0.30）%、（14.18±4.75）%、（6.61±1.06）%,差异有统计学意义（F=62.96Objective To investigate a brand new method for organ preservation in dry environment with high air pressure and to explore the possible mechanism of the method being superior to the commonly used method of soaking preservation by the detection of inflammatory factor. Methods From December 2014 to August 2015 a total of 60 SPF C57 BL /6 mice were enrolled including 36 donor mice of 4- 6 weeks and 24 recipient mice of 6- 8 weeks. The donor mice were divided into blank control group, histidine- tryptophan- ketoglutarate salt solution（ HTK） soaking preservation group and high pressure dry preservation group with 12 mice each group by random number table method. Recipient mice were divided into HTK solution preservation recipient group and high pressure dry preservation recipient group with 12 mice each group by random number table method. For blank control group, donor heart was taken away but not to be transplantated; the donor hearts of HTK soaking preservation group were soaked in HTK solution; the donor hearts of high pressure dry preservation group were hung in high pressure gas tank（ PO2： 1 600 h Pa ＋ PCO： 400 h Pa = 2 000 h Pa）. Two hours after transplantation,the donor heart re- beat rate and graft function score of HTK soaking preservation recipient group and high pressure dry preservation recipient group were compared and observed. Twenty- four hours after transplantation,RT- PCR method was used to detect the levels of TNF-α,IL-1β,IL-6,IL-10,ICAM- 1 and myocardium cell apoptosis index. Results The donor heart rebeat rate of high pressure dry preservation recipient group was higher than that of HTK soaking preservation recipient group 〔83. 3%（ 10 /12） vs. 25. 0%（ 3/12）,P = 0. 012〕. The donor heart graft function scores of high pressure dry preservation recepient group was higher than that of HTK soaking preservation recipient group 〔3. 5（ 1. 0） vs. 1. 3（ 0. 5）,Z =- 3. 447,P〈0. 01 〕. HTK soaking preservation group and high pressure dry preservation group were higher

关 键 词：器官保存 器官保存液 氧 一氧化碳 心肌再灌注损伤 肿瘤坏死因子Α 白细胞介素类 

分 类 号：R617[医药卫生—外科学]