浙江省不同地理株白纹伊蚊mtDNA-COⅠ基因多态性研究  

Genetic polymorphism analysis of cytochrome C oxidase subunit Ⅰ gene in Aedes albopictus from Zhejiang Province,China

作　　者：郭颂[1] 凌锋[1] 王金娜[1] 吴瑜燕[1] 侯娟[1] 龚震宇[1] 

机构地区：[1]浙江省疾病预防控制中心,杭州310051

出　　处：《中国人兽共患病学报》2016年第2期133-136,147,共5页Chinese Journal of Zoonoses

基　　金：国家十二五科技重大专项(No.2012ZX10004-219);2015年浙江省医药卫生平台计划(骨干人才A类)(No.2015RCA005)联合资助~~

摘　　要：目的比较浙江省不同地理区域白纹伊蚊种群线粒体细胞色素C氧化酶亚基Ⅰ(mtDNA-COⅠ)序列的多态性,探讨其遗传特征。方法采集登革热历史流行区和非流行区的白纹伊蚊雌性成蚊,PCR扩增COⅠ基因,测序后结合Genbank中获得的各地白纹伊蚊序列进行比对,分析基因特征和种群分化并构建系统进化树。结果用于分析的线粒体COⅠ基因长度为686bp,碱基A+T平均含量为67.8%,G+C平均含量为32.2%,变异位点中包括15个碱基转换位点和3个颠换位点。96个个体中存在20个单倍型,整体单倍型多样性为0.497,核酸多样性为0.717,核酸平均差异数为0.001 05。衢州、温州苍南和丽水的白纹伊蚊COⅠ序列多态性高于其它地区,且温州苍南和衢州白纹伊蚊种群与其它地区白纹伊蚊种群存在中等遗传分化。结论浙江省不同地理株白纹伊蚊COⅠ序列表现出一定程度的遗传多态性和的遗传分化,可能由环境、气候和人文因素综合所致。In order to study the characteristics of mitochondrial DNA Cytochrome C oxidase subunit I（mtDNA-COI）gene of Aedes albopictus from Zhejiang and to explore genetic polymorphism of this vector,adult female mosquitoes were collected from dengue epidemical and non-endemic areas from Zhejiang,and their COI genes were amplified by PCR and sequenced for genetic analysis including genetic feature,population differentiation and phylogenetic tree construction.The COIfragment contained 686 nucleotides with 18 polymorphic sites including 15 transition sites and 3transversion sites.The percentage of A＋T content in COI gene was 67.8%,while the percentage of G＋C was 32.2%.There were 20 haplotypes obtained from 96 specimens among 8geographic populations from Zhejiang Province.The overall haplotype diversity,nucleotide diversity and mean number of pairwise differences were 0.497,0.717 and 0.001 05,respectively.Aedes albopictus population from Cangnan,Quzhou and Lishui showed higher genetic polymorphism than other sampling sites,and Aedes albopictus population from Cangnan,Quzhou showed moderate differentiation with other populations.A certain degree of genetic polymorphism and genetic differentiation were found in Aedes albopictus populations from Zhejiang,which may be caused by some factors such as environment,climate and humanity.

关 键 词：白纹伊蚊 细胞色素C氧化酶亚基Ⅰ 多态性 

分 类 号：R384[医药卫生—医学寄生虫学]