贵州遵义地区蝙蝠脑组织BDV-P24基因片段的检测  

Borna disease virus p24 in brain tissue of bat infect in Zunyi,Guizhou Province,China

作　　者：杨利玲[1] 雷以会 徐平[1] 

机构地区：[1]遵义医学院第一附属医院神经内科,遵义563500

出　　处：《中国人兽共患病学报》2016年第2期156-159,共4页Chinese Journal of Zoonoses

基　　金：国家自然科学基金项目(No.31160210)资助~~

摘　　要：目的了解贵州遵义地区蝙蝠博尔纳病病毒(BDV)感染情况。方法采用巢式逆转录聚合酶链反应(nRTPCR)检测82例蝙蝠脑组织BDV-P24基因片段,阳性产物进行基因序列测定、同源性及分子系统树分析。结果在82例蝙蝠脑组织中检测出7例BDV-RNA P24基因片段阳性,只有5例成功测出BDV-P24基因片段,与标准株马源Strain V相比较同源性高达99%,出现2个位点的一致性沉寂突变(nt1650T^C,nt1740G-T,突变率为0.9%),与H1766比较其同源性达97%,出现5个位点的一致性沉寂改变(nt1599A^G,nt1671T^C,nt1677T^C,nt1695G^A,nt1740G^T,突变率为2.2%),与He/80比较起同源性为96%,出现7个位点的一致性沉寂改变(nt1566G^A,nt1581C^T,nt1659T^C,nt1668A^G,nt1674T^C,nt1695G^A,nt1740G^A,突变率为3.1%)。结论贵州遵义绥阳地区蝙蝠存在BDV感染,与马源Strain V存在高度同源性,人感染BDV可能存在潜在的动物源性。The p24 gene fragment of BDV-RNA in brain tissue of bat was detected by nested reverse transcriptase polymerase chain reaction（nRT-PCR）in Zunyi,Guizhou Province.The positive PCR products were analyzed by genetic sequence homology and molecular phylogenetic tree.Result showed that the P24 gene fragment of BDV-RNA in brain tissue from 82 bats was 7positived.There was only 5positive products could be successfully detected.The homology of p24 gene fragment of BDV was 99%,with 2situs consistency silent mutation when compared with standard strain V in horse（nt1650T-C,nt1740G-T,mutation rate of 0.9%）.The homology of p24 gene fragment of BDV was 97%,with 5situs consistency silent mutation when compared with H1766（nt1599A-G,nt1671T-C,nt1677T-C,nt1695G-A,nt1740G-T,mutation rate of 2.2%）.The homology of p24 gene fragment of BDV was 96%,with 7situs consistency silent mutation when compared with He／80（nt1566G-A,nt1581C-T,nt1659T-C,nt1668A-G,nt1674T-C,nt1695G-A,nt1740G-A,mutation rate of 3.1%）.There is BDV natural infection probably originated from bat,highly homology with strain V in horse in Zunyi,Guizhou Province.

关 键 词：博尔纳病病毒 蝙蝠 nRT-PCR P24 

分 类 号：R373[医药卫生—病原生物学]