子宫内膜异位症裸鼠模型子宫内膜的雌、孕激素受体表达  

Altered expression of progesterone receptor and estrogen receptor in eutopic endometrium in a nude mouse model of endometriosis

作　　者：冯雪[1] 宋永[1] 周敏[1] 肖丽[1] 朱慧莉[1] 黄薇[1] 

机构地区：[1]四川大学华西第二医院,成都610041

出　　处：《现代妇产科进展》2016年第2期112-115,119,共5页Progress in Obstetrics and Gynecology

基　　金：国家自然科学基金(No:81370693)

摘　　要：目的:建立子宫内膜异位症裸鼠模型,观察子宫内膜的雌激素受体(ERs)、孕激素受体(PRs)及其亚型的表达情况,为进一步探讨内异症子宫内膜孕激素抵抗的发生机制提供研究模型。方法:建立子宫内膜异位症裸鼠模型,分别于造模后1月、2月、3月取裸鼠子宫内膜,利用RT-qPCR和Western blot法检测其孕激素受体(PRA、PRB)和雌激素受体(ERα、ERβ)表达情况。结果:与对照组相比,内异症组裸鼠内膜的PRB和ERα表达降低,在造模后3月最明显;PR和ERβ表达升高,PRB/PRA和ERα/ERβ比值降低,均在造模后3月最明显。结论:子宫内膜异位症裸鼠模型是研究内异症的理想模型之一,造模后的裸鼠内膜存在孕激素抵抗。Objective： To observe gene and protein expression of progesterone receptor（ PRA,PRB） and estrogen receptor（ ERα,ERβ） in eutopic endometrium in a surgically-induced endometriosis nude mice model,and to explore a proper model for investigating the pathogenesis of progesterone resistance in endometriosis.Methods： Adult nude mice were surgically induced endometriosis by intraperitoneal inoculation of eutopic endometrium collected from infertile women with endometriosis.After 1,2,and 3 months,the endometrium of nude mice were collected.Then quantitative real-time RT-PCR and Western blot were used to analyze the gene and protein expression of PR（ PRA,PRB） and ER（ ERα,ERβ） in endometrium of experimental mice. Result： The expressions of PRB and ERα mRNA and protein statistically significant decreased at 3 month,while PR and ERβ expressions increased. The ratios of PRB / PRA and ERα / ERβ decreased,statistically significant at 3 month.Conclusions： Nude mouse model of endometriosis is the ideal model for the research of endometriosis,the endometrium of nude mouse exist progesterone resistance.

关 键 词：子宫内膜异位症 裸鼠 动物模型 孕激素抵抗 

分 类 号：R711.6[医药卫生—妇产科学]