鹿复合麻醉剂麻醉与大脑皮质部分ATP酶相关性研究  

Study on the correlation between the compound anesthetic for deer anesthesia and the partial ATPase in the cerebral cortex of Rats

作　　者：王楠[1] 付连军[1] 冯雪[1] 林博[1] 常乃元 李京乘 李闻轩[1] 尹柏双[1] 

机构地区：[1]吉林农业科技学院动物科技学院,吉林吉林132101

出　　处：《黑龙江畜牧兽医（下半月）》2016年第2期124-126,共3页

基　　金：国家自然科学基金项目(31302150);吉林省科技厅发展计划课题(20140204062NY);吉林省教育厅"十二五"科学技术课题(吉教科合字2014379);长白山动植物资源保护与利用省级重点实验室基金课题(吉农院合字2013S029);吉林农业科技学院博士启动基金课题(吉农院合字2012307);吉林农业科技学院种子基金课题(吉农院合字2013905);大学生科技创新项目(吉农院合字2014012)

摘　　要：为了探究鹿复合麻醉剂麻醉与脑区突触体ATP酶相关性,试验选取纯种SD大鼠32只,随机分为对照组、诱导组、麻醉组和催醒组,利用比色法测定样本中ATP酶活性。结果表明:药物作用后大鼠麻醉全程大脑皮质突触体Na^+-K^+-ATP和Mg^(2+)-ATP酶活性诱导组显著降低(P<0.05),麻醉组和催醒组极显著降低(P<0.01)。说明大脑皮质突触体Na^+-K^+-ATP和Mg^(2+)-ATP酶活性的抑制与鹿复合麻醉剂引起麻醉作用具有一定相关性。To investigate the correlation between the compound anesthetic for deer anesthesia and ATPase in brain synaptosomes, 32 pure - bred SD rats were randomly divided into a control group, an induction group, an anesthesia group, and a wake group. The activity of ATPase in the samples was determined using colorimetric assay. The results showed that the activities of Na＋ - K ＋ - ATPase and Mg2 ＋ - ATPase from synap- tosomes in the cerebral cortex of rats during anesthesia after administration in the induction group were significantly decreased （ P 〈 0.05 ）, which were highly significantly decreased （ P 〈 0.01 ） in the anesthesia group and the wake group. The results indicate that the inhibition of the activities of Na＋ - K＋ - ATPase and Mg2 ＋ - ATPase from synaptosomes in the cerebral cortex has a certain correlation with the anesthesia effect of the compound anesthetic for deer.

关 键 词：鹿复合麻醉剂 鹿 大脑皮质 NA+-K+-ATP酶 Ca2+-Mg2+-ATP酶 

分 类 号：S857.124[农业科学—临床兽医学] S835[农业科学—兽医学]