气相色谱法测定冰霜梅苏丸中龙脑、异龙脑、薄荷脑的含量  

Determination of borneol, isoborneol and menthol in Bingshuang-Meisu pill by GC

作　　者：周雪红[1] 李志浩 

机构地区：[1]湖北医药学院附属东风医院药学部,十堰442008 [2]武当特色中药研究湖北省重点实验室

出　　处：《国际中医中药杂志》2016年第3期250-253,共4页International Journal of Traditional Chinese Medicine

基　　金：武当特色中药研究湖北省重点实验室开放课题项目（WDCM006）;湖北省2011协同创新中心基金（4）

摘　　要：目的建立测定冰霜梅苏丸中龙脑、异龙脑、薄荷脑含量的气相色谱方法。方法以PEG-20M为固定相的毛细管色谱柱（30m×0．32mm，0．25μm）进行测定，以萘为内标，N：为载气，分流进样（分流比10：1），程序升温，进样口温度240℃，检测器温度250℃。结果薄荷脑、龙脑、异龙脑分别在13．00～104．00μg／ml、6．80-54．40μg／ml、5．40-43．20μg／ml浓度范围内线性关系良好，加样回收率分别为97．90％、98．05％、97．77％，RSD分别为O．98％、1．53％、1．40％。结论本方法灵敏，准确，重复性好，可用于冰霜梅苏丸的质量控制。Objective To establish a method for the determination ofborneol, isobomeol, menthol in Bingshuang-Meisu pill. Methods The capillary column PEG-20M（30 m × 0.32 mm, 0.25 μm） was used, naphthalene was adopted as internal standard substances, split injection（split ratio 10：1）, temperature programme, the injector temperature was 240 ~C, the detector temperature was 250 ℃. Results The linear response range of menthol, bomeol, isobomeol were 13.00-104.00 ~tg/ml, 6.80-54.40 μg/ml, 5.40-43.20 μg/ml and the average recovery were 97.90%, 98.05% and 97.77%, RSD were 0.98%, 1.53% and 1.40% respectively. Conclusion The method was highly sensitive, accurate, and repeatable. It can be an important method to control the standard of Bingshuang-Meisu pill.

关 键 词：色谱法 气相 龙脑 薄荷脑 冰霜梅苏丸 

分 类 号：O657.71[理学—分析化学] R286.0[理学—化学]