苦豆子总碱对糖尿病性膀胱病大鼠逼尿肌活动的影响  

Effects of total alkaloid of sophora alopecuroids on bladder detrusor muscle activity in diabetic cystopathy rats

作　　者：李妍 孙安 牛彩琴[3] 

机构地区：[1]静宁县人民医院,甘肃平凉743400 [2]兰州军区空军机关医院特诊科,甘肃兰州730020 [3]川北医学院中西医临床医学系,四川南充637007

出　　处：《现代医药卫生》2016年第6期811-813,816,共4页Journal of Modern Medicine & Health

基　　金：四川省教育厅重点项目(14ZA0182)

摘　　要：目的观察苦豆子总碱(TASa)对糖尿病性膀胱病(DCP)大鼠膀胱逼尿肌活动的影响。方法 (1)采用链脲佐菌素法建造糖尿病模型;(2)测定尿流动力学指标确立DCP模型;(3)采用恒温灌流浴槽,将大鼠膀胱逼尿肌离体肌条标本分为对照组(正常肌条,12份)和DCP模型组(DCP肌条,72份),再将DCP模型组分为TASa组、TASa联合异搏定(维拉帕米)组、TASa联合酚妥拉明组、TASa联合苯海拉明组、TASa联合消炎痛(吲哚美辛)组和TASa联合阿托品组,各12份。探讨TASa对DCP逼尿肌自发收缩的影响。结果 TASa能增强DCP膀胱逼尿肌自发收缩的振幅,并具有量效依赖性(r=0.89,t=3.39,P<0.05);用线性回归法计算半数有效浓度为23.79 mg/L;但对频率[DCP模型组为(4.48±0.17)次/分,TASa组为(4.50±0.09)次/分]无明显影响,差异无统计学意义(P>0.05);异搏定能明显抑制TASa收缩膀胱逼尿肌的作用,而阿托品、酚妥拉明、苯海拉明和消炎痛无影响。结论 TASa可能是激活钙离子通道而增加DCP大鼠膀胱逼尿肌的收缩。Objective To observe the influence of the total alkaloid of sophora alopecuroids L（TASa） on bladder detrusor muscle activity in diabetic cystopathy（DCP） rats. Methods（1）The diabetic rat model was established by adopting streptozotocin（STZ）;（2）the urodynamic indicators were detected for establishing the DCP model;（3）the constant temperature perfusion bath tank was adopted to divide the isolated bladder detrusor muscle strips into the control group（normal muscle strip,12 samples）and DCP model group（DCP muscle strip,72 samples）,then,the DCP group was subdivided into the TASa group,TASa combined verapamil group,TASa combined phentolamine group,TASa combined indometacin group and TASa combined with atropine group,12 samples in each group. The influence of TASa on the spontaneous contraction of DCP detrusor muscle was investigated.Results TASa could increase the contraction amplitude of bladder detrusor musclen with dose-effect dependence（r=0.89,t=3.39,P〈0.05）. The 50% effective concentration（EC50） calculated by using the linear regression method was 23.79 mg/L;but which had no influence on frequency[（4.48±0.17） times/min in DCP model group,（4.50±0.09） times/min in TASa group],the difference was not statistically significant（P〉0.05）. Verapamil could significantly inhibit the TASa action for contracting bladder detrusor muscle,but atropine,phetolamine,benzhydramine and indomethacin had no influence. Conclusion TASa may activate calcium ion channel to increase contraction of bladder detrusor muscle.

关 键 词：苦豆子 生物碱类 膀胱疾病 糖尿病并发症 尿动力学 逼尿肌 

分 类 号：R587.2[医药卫生—内分泌]