斜投影和空间夹角判据结合紫外光谱分析滴眼液中苯扎氯铵  

Rapid Determination of Benzalkonium Chloride in Eye Drops with Ultraviolet Spectrum Based on Oblique Projection and Space Angle Criterion

作　　者：杨向妮[1,2] 姚志湘[1,2] 粟晖[1,2] 刘柳[1,2] 

机构地区：[1]广西科技大学生物与化学工程学院,广西柳州545006 [2]广西糖资源绿色加工重点实验室,广西柳州545006

出　　处：《分析测试学报》2016年第3期337-341,共5页Journal of Instrumental Analysis

基　　金：广西科技计划项目(桂科攻1355010-15);柳州市科技计划项目(2015J030201)

摘　　要：采用紫外光谱,将斜投影（OP）与空间夹角判据（SAC）相结合,通过十二烷基二甲基苄基氯化铵（1227）含量反映苯扎氯铵（BAC）含量的方式建立了一种快速检测滴眼液中BAC的新方法。首先采用斜投影提取BAC混合物中1227的纯信号,以提取的光谱信号与对应浓度值进行最小二乘拟合,建立标准光谱矩阵;再通过高效液相色谱-紫外光谱联用获取滴眼液本底光谱矩阵;最后根据空间夹角判据测定滴眼液样品中1227的含量,并以此反映BAC的含量。结果显示：在1-20μg/mL浓度范围内,斜投影提取的1227纯信号与对应浓度呈良好线性关系（r〉0.999 6）,方法的检测结果与高效液相色谱法接近,相对误差不大于2.3%,相对标准偏差（RSD,n=6）不大于2.8%,回收率为99.6%-101.8%。该方法采用多波长紫外光谱直接定量BAC,无需复杂的样品前处理,定量时间短,分析效率高,稳健性好,可推广应用于其他领域。A rapid new method for the determination of benzalkonium chloride（ BAC） in eye drops complex system was developed with ultraviolet spectrum based on oblique projection（ OP） and space angle criterion（ SAC）,in which the content of BAC is based on content of dodecyl dimethyl benzyl ammonium chloride（ 1227）. By treatment with oblique projection,the pure spectrum of dodecyl dimethyl benzyl ammonium chloride was obtained. The standard spectral matrix was set up by the least squares fitting the spectrum with the corresponding concentration. And then the background spectral matrix was obtained by using high performance liquid chromatography- ultraviolet spectrum（ HPLC- UV）. After that the contents of 1227 in different kinds of eye drops were calculated by using space angle criterion,and the content of BAC was obtained. The results indicated that the method exhibited a good linearity（ r 〉0. 999 6） for BAC in the range of 1- 20 μg / mL. The determination results of this method is close to that of HPLC method,and the relative error is not more than2. 3%. The recoveries were obtained between 99. 6% and 101. 8%,with RSDs not more than 2. 8%.The content of BAC could be directly obtained with multiple wavelength ultraviolet spectrum without complicated sample preparation,and the method has the advantages of short quantitative time,high analysis efficiency,good robustness,and could provide a feasible scheme for the detection of analyte of interest in other complex system.

关 键 词：斜投影 空间夹角判据 高效液相色谱-紫外光谱联用 苯扎氯铵 滴眼液 

分 类 号：O657.3[理学—分析化学] S482.294[理学—化学]