花生干旱及高盐胁迫下的酵母双杂交文库构建  

Construction and identification of a yeast two-hybrid cDNA library of peanut under drought and high salinity stress

作　　者：刘永惠[1] 沈一[1] 陈志德[1] 

机构地区：[1]江苏省农业科学院经济作物研究所,江苏南京210014

出　　处：《江苏农业学报》2016年第1期40-43,共4页Jiangsu Journal of Agricultural Sciences

基　　金：国家现代农业花生产业技术体系项目(CARS-14);江苏省农业科技自主创新基金项目[CX(14)2001]

摘　　要：干旱、高盐等胁迫对花生的产量和品质影响较大,为了挖掘花生耐干旱和高盐胁迫相关基因,并阐释其编码蛋白质间的互作关系,本研究利用SMART技术,构建了花生干旱及高盐胁迫下的酵母双杂交文库。结果表明,构建的文库容量为1.26×106CFU,文库滴度达到1.07×108CFU/ml,从随机挑选的16个克隆的PCR结果来看,插入片段大小差异明显,主要分布在500~2 000 bp,重组率为100%。说明该文库具有较高的质量,可以用于进一步的筛选工作。Yield and quality of peanut（ Arachis hypogaea L.） were affected by water stress such as drought and high salinity. In order to mine genes related to water stress tolerance in peanut,and describe the interaction among encoded proteins,a yeast two-hybrid cDNA library of peanut under drought and high salinity stress was constructed by SMART（ switching mechanism at 5＇ end of the RNA transcript） technique. The results showed that the average capacity of the cDNA library was about 1.26× 106 independent clones,and the titer was up to 1.07× 108 CFU / ml. The PCR amplification of 16 clones randomly selected from the cDNA library revealed that the lengths of inserted cDNA fragments ranged from 500 bp to2 000 bp,with the recombination rate of 100%. It is indicated that the cDNA library was constructed with a high quality,and was applicable for screening interaction proteins.

关 键 词：花生 干旱 高盐 酵母双杂交cDNA文库 

分 类 号：S565.2[农业科学—作物学]