淫羊藿醇提物抑制肺癌生长及调节免疫作用研究  

Effect of Epimedii Herba alcohol extract on inhibition of lung tumor growth and immunomodulatory

作　　者：袁嘉瑞 汪春飞 宋捷[3] 赵冰洁[3] 董自波 封亮[3] 贾晓斌[1] 

机构地区：[1]南京中医药大学附属中西医结合医院,江苏南京210028 [2]济川药业集团有限公司,江苏泰兴225441 [3]江苏省中医药研究院国家中医药管理局中药释药系统重点研究室,江苏南京210028

出　　处：《中国中药杂志》2016年第1期112-117,共6页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金青年基金项目(81303275);苏州市2012年科技计划医疗器械与新医药专项(ZXY2012009)

摘　　要：该实验采用C57BL/6小鼠Lewis肺癌模型,观察淫羊藿醇提物(HE)对C57BL/6小鼠Lewis肺癌生长的影响,研究其抗肺癌效果及免疫调节能力;同时通过ELISA法检测血清中TNF-α,IL-10,IL-17,IL-2水平;并采用免疫组织化学法和蛋白免疫印迹法检测肺癌组织中Ki67和P53蛋白的表达。结果表明淫羊藿醇提物对C57BL/6小鼠Lewis肺癌的肿瘤生长具有一定的抑制作用,且能够降低血清中TNF-α,IL-10和IL-17的水平,提高IL-2的水平,并且能使得Ki67的表达显著降低,P53的表达显著增强。淫羊藿醇提物对肺癌具有明显的抑制作用,并且具有一定的提高免疫调节能力的作用,揭示了淫羊藿醇提物的抗肺癌作用可能与其提高免疫调节能力有关,从而为淫羊藿醇提物抗肺癌作用的进一步研究提供了基础。To observe the effect of Epimedii Herba alcohol extract（ HE） on tumor growth of lung cancer by establishing the model of Lewis tumor-bearing mice,ELISA method was used to detect the levels of TNF-α,IL-10,IL-17,IL-2 in serum. Ki67 and P53 protein expression was detected in lung cancer tissues by using Western blot assay method and immunohistochemical assay method. The experimental results showed that HE has certain inhibitory effect on Lewis lung cancer tumor growth,and it can reduce the levels of TNF-α,IL-10 and IL-17 in serum,improve the level of IL-2,significantly decrease the expression of Ki67,and significantly increase P53 expression. HE has obvious inhibitory effect against lung cancer,and has the ability to improve immune regulating effect. This study reveals the anti-lung cancer effect of HE may be related to its ability of improving immunity,thus provides the basis for further research on anti-lung cancer effect of HE.

关 键 词：淫羊藿 肺癌 抑制肿瘤生长 免疫调节 

分 类 号：R285[医药卫生—中药学]