NaCl胁迫下转Bt棉花和非转Bt棉花的生理反应差异  

Physiological Responses Difference of Transgenic Bt Cotton and Nontransgenic Bt Cotton under the Stressing of NaCl

作　　者：朱永红[1] 潘转霞[1] 夏芷 赵俊侠[1] 

机构地区：[1]山西省农业科学院棉花研究所,山西运城044000

出　　处：《山西农业科学》2016年第3期297-299,共3页Journal of Shanxi Agricultural Sciences

基　　金：棉花生物学国家重点实验室开放课题(CB2015A04);山西省财政厅种业发展专项(2015ZYZX-06);山西省科技基础条件平台建设项目(20150910004-0103)

摘　　要：为研究NaCl胁迫下转Bt基因棉花和非转Bt基因棉花的生理反应差异,采用盆栽条件下不同浓度NaCl溶液浇灌处理,测定叶片细胞含水量(RWC)、丙二醛(MDA)含量、游离脯氨酸(Pro)含量和Bt毒素蛋白含量。结果表明,NaCl胁迫下转Bt基因棉花和非转Bt基因棉花叶片细胞含水量比无胁迫明显降低,丙二醛含量明显升高,游离脯氨酸含量大幅增加,这些生理变化在转Bt基因棉花和非转Bt基因棉花之间没有显著差异,说明转入Bt基因对棉花的生长发育不会造成显著影响;在0.4%NaCl胁迫下,转Bt基因棉花Bt毒素蛋白含量出现了显著下降,说明在NaCl胁迫下转Bt基因棉花的抗虫性下降。To seek whether there was any difference on physiological reactions between transgenic Bt cotton and non-transgenic Bt cotton under the stressing of NaCl, the cell water content, malondialdehyde（MDA）, free proline（Pro）and Bt toxin protein of leaf cells were measured on the condition of potted cotton irrigated with different concentrations of NaCl. The results showed that the cell water content decreased significantly under NaCl stress in cotton leaf cells, malondialdehyde（MDA）content increased significantly, proline（Pro）content increased significantly, and there was no significant difference in transgenic Bt cotton and non transgenic Bt cotton. It indicated that transferred Bt gene on cotton didn＇t affect the growth and development of cotton. The Bt toxin protein content of transgenic Bt cotton appeared significantly decreased. It indicated that the resistance of cotton bollworm decreased under NaCl stressing.

关 键 词：NACL胁迫 转BT基因棉花 非转Bt基因棉花 生理反应 毒素蛋白 

分 类 号：S562[农业科学—作物学]