黄瓜内生真菌对根结线虫生防活性测定  

Biocontrol against Meloidogyne incognita by an Endophytic Fungus Plectosphaerella sp. from Cucumber Root Galls

作　　者：李莹[1] 秦明一[2] 张超[2] 王殿东[2] 田雪亮[3] 

机构地区：[1]北华大学,吉林吉林132013 [2]长江师范学院,重庆408100 [3]河南科技学院,河南新乡453003

出　　处：《湖北农业科学》2016年第3期634-637,共4页Hubei Agricultural Sciences

基　　金：吉林省杰出青年培养专项(2014-201464040);重庆市教委项目(kj1401213);国家自然科技基金青年科学基金项目(31101418)

摘　　要：为了明确黄瓜根结内生真菌对根结线虫(Meloidogyne incognita)的防效,采用菌株发酵液对根结线虫进行离体活性和盆栽防效测定,结果表明,菌株经形态和18S r DNA鉴定为Plectosphaerella sp.,其发酵液对根结线虫处理48 h的致死活性为93.73%;盆栽试验表明,Plectosphaerella sp.对根结线虫的防效为56.1%。发酵液处理的黄瓜根系卵块数量少于对照,土壤中根结线虫2龄幼虫数量也少于对照。总体来看,Plectosphaerella sp.对根结线虫具有较高的生防活性,能为黄瓜根结线虫生物防治奠定基础。To assess bioncontrol activity against root knot nematode by an endophytic fungus isolated from cucumber root galls,the biocontrol potential of the fungus was analyzed through in vitro test and pot experiment. The fungus was identified as Plectosphaerella sp. according to morphological characteristics and 18 S r DNA sequences. The fungal fermentation broth killed 93.73% of root knot nematode for 48 h. The control efficiency of Plectosphaerella sp. revealed by pot experiment was56.1%. The number of egg mass on cucumber root and population of root knot nematode in soil treated by fungus were higher than that by control. In a conclusion,the fungus possessed high biocontrol activity against root knot nematode.

关 键 词：黄瓜 内生真菌 生物防治 根结线虫(Meloidogyne incognita) 

分 类 号：S432.44[农业科学—植物病理学]