四种柱花草种子萌发中参与氮代谢的酶活性变化  

Study on the Changes of Enzyme Activity Involved in the Nitrogen Metabolism of Four Kinds of Stylo Seed Germination

作　　者：张瑜[1] 严琳玲[1] 虞道耿[1] 杨虎彪[1] 白昌军[1] 

机构地区：[1]中国热带农业科学院热带作物品种资源研究所/农业部热带作物种质资源利用重点开放实验,海南儋州571737

出　　处：《湖北农业科学》2016年第3期695-698,共4页Hubei Agricultural Sciences

基　　金：中国热带农业科学院热带作物品种资源研究所基本科研业务费(pzs1630032013015);热带作物种质资源平台项目;农业部物种资源保护项目

摘　　要：研究了4种柱花草种子萌发过程中参与氮代谢的蛋白酶、肽酶和硝酸还原酶活性的动态变化。结果表明,蛋白酶的活性随着柱花草种子萌发的深入而不断上升,在种子即将露白胚根突破种皮的时候蛋白酶活性达到最大值。此后,随着胚芽的生长蛋白酶活性逐渐降低,其中热研2号柱花草的蛋白酶活性高于其他3种;肽酶活性的变化总体呈上升趋势;硝酸还原酶呈S型变化。了解柱花草种子萌发期各种酶活性的变化规律,为深入研究柱花草种子萌发时贮藏物质的动员和代谢机理提供数据。This paper studied the dynamic change of protease, peptidase and nitrate reductase activity involved in nitrogen metabolism of four kinds of Stylos seed germination process, the results showed protease activity increased with Stylo seed germination, reached a maximum when the radicle breakthrough seed coat.Thereafter, protease activity decreased gradually with the growth of germs, protease activity of Stylo cv. Reyan NO.2 was higher than the others.The overall change in peptide activity showed increasing trend. Nitrate reductase was S shape change. Understood the variation rule of the various enzyme activity during seed germination of Stylo, to provide data for further study on the mechanism of mobilization and metabolism during Stylo seeds germination of storage substances.

关 键 词：柱花草[Stylosanthes guianensis(Aubl)SW.] 种子萌发 氮代谢 酶活性 

分 类 号：S54[农业科学—作物学]