HPLC测定复方足癣乳膏中甲硝唑含量  

Determination of the Metronidazole in Tinea pedis Cream by HPLC

作　　者：陈娟[1] 徐剑[1] 张永萍[1] 

机构地区：[1]贵阳中医学院,贵州贵阳550002

出　　处：《贵阳中医学院学报》2016年第2期29-31,共3页Journal of Guiyang University of Chinese Medicine

基　　金：成都军区医学科学技术研究"十二五"计划项目;项目编号:No:C12004

摘　　要：目的：建立高效液相色谱法测定复方足癣乳膏中甲硝唑的含量。方法：色谱柱：DIKMA Diamonsil C18柱（250×4.6mm,5μm）,流动相：甲醇-水（20：80）;流速：1.0m L·min^-1,柱温：30℃,检测波长：320nm。结果：甲硝唑在3.148μg.m L-1~37.78μg.m L^-1范围内线性关系良好（r=0.9995）,平均回收率100.80%,RSD=2.5%。结论：本方法结果准确,专属性强,重复性好,可用于复方足癣乳膏的含量测定方法。Objective： To establish an HPLC method for the determination of metronidazole in Tinea pedis cream. Method： The column was D1KMA DiamonsilC18 （250mm×4.6mm,5μm）. The mobile phase was methanol- water（20：80）. The flow rate was 1.0 mL ·min^-1 The detection wavelength was 320nm and the temperature of column was 30℃. Result： The metronidazole showed a good linearity in the range of 3. 148 - 37.78μg. mL^-1, r =0. 9995. The average recovery was 100. 80%, and RSD was 2. 5%. Conclusion： This method is simple, accurate and reliable, and can be used for the aualitv of Tinea pedis cream.

关 键 词：高效液相色谱法 含量测定 复方足癣乳膏 甲硝唑 

分 类 号：R927.2[医药卫生—药学]