天府肉鸭发育过程中胸腺Bcl-2,Bax基因及蛋白的表达  

Expression of mRNA and proteins of Bcl-2 and Bax during the development in duck thymus

作　　者：刘春雨[1] 朱盼盼[1] 方静[1] 蒋敏[1] 尹恒[1] 

机构地区：[1]四川农业大学动物医学院,四川雅安625014

出　　处：《浙江农业学报》2016年第1期15-21,共7页Acta Agriculturae Zhejiangensis

基　　金：教育部长江学者和创新团队发展计划(0848)

摘　　要：为探讨胸腺淋巴细胞凋亡的调控机制,将65只天府肉鸭分为22,24,26 d胚龄3个组和胚后0（新生雏）,3,5,8,14,17,20,26,29,32周龄10个组,采用免疫组织化学和原位杂交方法研究天府肉鸭胚胎及胚后发育期胸腺中Bcl-2和Bax mRNA及其蛋白的表达情况。结果表明,Bcl-2和Bax mRNA及蛋白免疫阳性反应见于胸腺淋巴细胞及胸腺上皮细胞。胸腺小叶皮质和髓质淋巴细胞BCL-2蛋白阳性率在22 d胚龄-17周龄恒定,20-32周龄呈下降趋势;淋巴细胞BAX蛋白阳性率在22-26 d胚龄恒定,0-8周龄上升,8-17周龄恒定,20-32周龄呈上升趋势;淋巴细胞BCL-2/BAX值在22-26 d胚龄恒定;0-8周龄下降,8-17周龄恒定,20-32周龄下降。胸腺皮质和髓质淋巴细胞Bcl-2 mRNA和Bax mRNA阳性率分别与其蛋白阳性率变化规律一致。各组皮质淋巴细胞Bcl-2 mRNA与蛋白阳性率以及BCL-2/BAX值均显著低于髓质;各组皮质淋巴细胞Bax mRNA和蛋白阳性率均明显高于髓质。推测BCL-2和BAX蛋白及其基因是天府肉鸭胸腺胚胎及胚后发育期淋巴细胞凋亡的重要调节物。In order to study the expression of Bcl-2 and Bax mRNA and their proteins during the embryonic and post embryonic development of Tianfu duck thymus,65 Tianfu ducks were divided into 3 groups as follows： 22,24 and26 days at embryonic stage,and 0（ neonatal stage）,3,5,8,14,17,20,26,29 and 32 weeks after hatching. Immunohistochemisty and in situ hybridization techniques were used in this study. The results showed that Bcl-2 and Bax mRNA and their proteins were distributed in the thymic lymphocytes and epithelial cells. The BCL-2 positive ratios of lymphocytes in the thymic cortex and medulla were not changed from 22 days at embryonic stage to 17 weeks after hatching,but were decreased from 20 to 32 weeks after hatching. The BAX positive ratios of lymphocytes in the thymic cortex and medulla were kept unchanged from 20 to 26 days at embryonic stage and from 8 to 17 weeks afterhatching,but were increased from 0 to 8 weeks and from 20 to 32 weeks after hatching. The BCL-2 / BAX values of lymphocytes in the thymic cortex and medulla were not changed from 22 to 26 days at embryonic stage and from 8 to17 weeks after hatching,but were decreased from 0 to 8 weeks and from 20 to 32 weeks after hatching. The change patterns of Bcl-2 mRNA and Bax mRNA positive ratios of lymphocytes in the thymic cortex and medulla were similar to their protein positive ratios,respectively. In each group,the BCL-2 protein,Bcl-2 mRNA positive ratios and BCL-2 / BAX value of lymphocytes in the follicle cortex were significantly lower than those in the medulla,while the BAX protein and Bax mRNA positive ratios of lymphocytes in the follicle cortex were significantly higher than those in the medulla,respectively. This study indicated that Bcl-2,Bax genes and their proteins are important modulators for the lymphocyte apoptosis of Tianfu duck thymus.

关 键 词：鸭 胸腺 Bcl-2 BAX 免疫组化 原位杂交 

分 类 号：S834.89[农业科学—畜牧学] S852[农业科学—畜牧兽医]