热应激对猪卵母细胞葡萄糖-6-磷酸脱氢酶活性及染色质构型的影响  

Effects of heat stress on glucose-6-phosphate dehydrogenase activity and the chromatin configuration in pig oocytes

作　　者：刘勇[1] 张领[1] 吴晓庆[1] 卫朝辉 王艳红[1] 王启磾 高迪[1] 丁彪[1] 吴风瑞[1] 王荣[1] 李文雍[1] 

机构地区：[1]阜阳师范学院胚胎发育与生殖调节安徽省重点实验室,安徽阜阳236037

出　　处：《浙江农业学报》2016年第1期22-27,共6页Acta Agriculturae Zhejiangensis

基　　金：国家自然科学基金(31201789;31372273);安徽省自然科学基金项目(1508085QC59;1408085QC65);安徽省省级学科建设重大项目(皖教秘科[2014]28号);安徽省大学生创新创业训练计划项目(AH201410371031);省级科研机构校级委托专项(2014PTFY04ZD);阜阳师范学院学生科研立项资助的课题(fsyxsc201431)

摘　　要：将屠宰场采集的猪卵巢分为热应激组和对照组,分别检测两组未成熟卵母细胞的各项指标。利用亮甲酚蓝染色法研究葡萄糖-6-磷酸脱氢酶活性,对比分析热应激对细胞代谢活性的影响;利用Hoechst33342标记DNA区分染色质构型,对比分析热应激对细胞染色质构型的影响。结果表明:热应激组卵母细胞BCB+比例显著低于对照组(P<0.05)。热应激组卵母细胞的各期染色质构型比例与对照组比例差异不显著(P>0.05)。BCB+和BCB-组卵母细胞GV1-GV3染色质构型差异不显著(P>0.05),但对照组BCB+卵母细胞的GV4构型比例明显高于BCB-(P<0.05),且GVBD差异不显著(P>0.05);而热应激组BCB+卵母细胞的GVBD比例明显低于BCB-(P>0.05),且GV4差异不显著(P>0.05)。综上,热应激对猪未成熟卵母细胞造成的损伤主要是影响了细胞的代谢活性,对细胞染色质构型的影响不大。Pig ovaries collected from the slaughterhouse were divided into heat stress group and control group. Immature oocytes were taken from ovaries in each group. Brilliant cresol blue staining method was used to study glucose-6-phosphate dehydrogenase（ G6PD） activity of immature oocytes in each group. Hoechst 33342 was used to label DNA and distinguish the chromatin configuration. It was shown that the percentage of BCB ＋ oocyte in heat stress group was significantly lower than that in control group（ P〈0. 05）. The proportions of chromatin configuration exhibited no significant differences between heat stress group and control group（ P〉0. 05）. The proportions of GV1 to GV3 hadno significant difference between BCB ＋ and BCB-oocytes（ P〉0. 05）. In the control group,the proportion of GV4 chromatin configuration was obviously higher in BCB ＋ oocytes than that in BCB- ones（ P〈0. 05）,but the proportion of GVBD had no significant difference（ P〉0. 05）. The proportion of GV4 chromatin configuration had no significant difference between BCB ＋ and BCB- in the heat stress group（ P〉0. 05）,but the proportion of GVBD was obviously lower in BCB ＋ oocytes than that in BCB-（ P〈0. 05）. In conclusion,the heat stress affected the metabolic activity of porcine immature oocytes,but had little effects on chromatin configuration.

关 键 词：染色质构型 葡萄糖-6-磷酸脱氢酶 猪卵母细胞 热应激 

分 类 号：S811.2[农业科学—畜牧学]