栀子苷对通过TLR4-NF-κB信号通路抑制缺氧/复氧心肌细胞前炎症因子释放的影响  

Inhibitory Effect of Geniposide on Release of Pro-inflammatory Cytokines of Anoxia/reoxygenation Myocardial Cells Through TLR4-NF-κB Signaling Pathway

作　　者：蔡智慧[1] 王晋[1] 郑亚萍[1] 

机构地区：[1]漯河医学高等专科学校生理学教研室,河南漯河462002

出　　处：《广州中医药大学学报》2016年第2期234-237,共4页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：河南省高等专校重点科研项目(编号:16A180031)

摘　　要：【目的】探讨栀子苷对缺氧/复氧心肌细胞前炎症因子肿瘤坏死因子-α(TNF-α)、白细胞介素-1(IL-1)、IL-6释放以及对Toll样受体4(TLR4)和活化核因子-κB(NF-κB)蛋白表达的影响。【方法】原代培养SD大鼠乳鼠心肌细胞。实验分为正常对照组、缺氧/复氧模型组和栀子苷预处理组,采用酶联免疫吸附(ELISA)法检测细胞培养液前炎症因子TNF-α、IL-1、IL-6的含量,Western blot法检测心肌细胞TLR4、NF-κB蛋白表达。【结果】与缺氧/复氧模型组比较,栀子苷预处理组心肌细胞TNF-α、IL-1、IL-6释放显著减少,TLR4、NF-κB蛋白表达显著下降,差异均有统计学意义(P<0.05或P<0.01)。【结论】栀子苷可抑制缺氧/复氧心肌细胞前炎症因子TNF-α、IL-1、IL-6释放,其作用机制可能与栀子苷下调TLR4-NF-κB通路有关。Objective To explore the effect of geniposide on the release of pro-inflammatory cytokines tumor necrosis factor-alpha（TNF-α）,interleukin（IL）-1 and IL-6 in hypoxia/reoxygenation myocardial cells and on the expression of Toll- like receptor（TLR） 4 and nuclear factor-kappa B（NF-κB） of the myocardial cells.Methods Primary rat cardiac myocytes were cultured. And the cells were randomly divided into 3 groups,namely normal control group, hypoxia/reoxygenation group and geniposide-pretreatment group. The contents of TNF-α,IL-1,and IL-6 were determined by enzyme-linked immunosorbent assay（ELISA）,and the protein expression levels of TLR4 and NF-κB were detected by Western blotting method. Results Compared with the hypoxia/reoxygenation group,the geniposide-pretreatment group reduced the release of TNF-α, IL-1, IL-6（P〈0.05） and decreased the expression of TLR4 and NF-κB（P〈0.05 or P〈0.01）. Conclusion Geniposide can inhibit the release of pro-inflammatory cytokines in the anoxia/reoxygenation myocardial cells, and its mechanism may be associated with the down-regulation of TLR4-NF-κB signaling pathway.

关 键 词：栀子苷/药理学 心肌缺血/中药疗法 缺氧/复氧 前炎症因子 蛋白表达 细胞培养 疾病模型 动物 大鼠 

分 类 号：R285.5[医药卫生—中药学]