产玫瑰红色素粘质沙雷氏菌的分离鉴定及抑菌活性  

Isolation,identification and antibacterial activities of Serratia marcescens producing rosy pigment

作　　者：董婷[1] 景波[1,2] 李伟[1] 黄晓雅[1] 钱俏君 杨慧[1] 潘康成[1,2] 

机构地区：[1]四川农业大学动物医学院动物微生态研究中心,四川成都611130 [2]动物疫病与人类健康四川省重点实验室,四川成都611130

出　　处：《浙江农业学报》2016年第2期252-258,共7页Acta Agriculturae Zhejiangensis

基　　金："教育部长江学者和创新团队发展计划"创新团队资助项目(IRT0848);四川农业大学教学改革研究项目(X2011008)

摘　　要：旨在分离鉴定产玫瑰红色素菌株,及其分离菌株和色素对病原菌的生物拮抗作用。经形态观察、生理生化试验和16S r RNA序列分析对其进行鉴定,通过控制变量法确定最佳产色素的温度及光照条件,破裂菌体提取色素分析此菌株产色素的量和红色素的性质,采用滤纸片法研究对病原菌的拮抗作用。结果表明：（1）分离细菌Dse-01菌株最终鉴定为粘质沙雷氏菌（Serratia marcescens）;（2）该菌在25-35℃条件下培养色素形成较早且颜色较深,30℃温度下产色素最佳,光照对其色素形成时间没有明显影响;（3）30℃150 r·min^-1培养24 h,提取获得色素粗品确定为灵菌红素,产量达（567.90±7.77）mg·L^-1;（4）分离菌株的全菌液、上清液、菌体和提取色素对病原性大肠杆菌、沙门氏菌均无拮抗作用,而全菌液、菌体和提取色素对金黄色葡萄球菌具有明显的抑制作用。This experiment was conducted to isolate and identify a microbial strain producing rosy pigment,and to study its antibacterial activity. The microbial strains were identified by morphological characteristics,physiochemical properties and 16 S r RNA gene sequence analysis. The optimization of temperature and illumination condition for rosy pigment production was investigated by variable-controlling approach. Ultrasonic fragmentation was used to extract pigment and analyze its yield and absorption spectrum. In eventually,antagonism assay to pathogens also be carried out by filter-paper method. The results showed that：（ 1） a screened strain Dse-01 was identified as Serratia marcescens;（ 2） The strain showed significant impact on chromogenesis under the condition of 25- 35 ℃,especially at30 ℃,which was not affected by illumination;（ 3） The strain was cultured at 30 ℃ for 24 h with a shaking speed of150 r·min^- 1and the extracts was prodigiosin,whose production could reach（ 567. 90 ± 7. 77） mg·L^- 1;（ 4） the pathogenic Escherichia coli and Salmonella spp. were resistant to four active substance： Serratia marcescens,sediment of Serratia marcescens,supernatant of Serratia marcescens and pigment extraction from Serratia marcescens,whereas,Staphylococcus aureus was sensitive to all of these active products except the supernatant.

关 键 词：粘质沙雷氏菌 灵菌红素 生物拮抗 

分 类 号：S816.79[农业科学—饲料科学] Q939.92[农业科学—畜牧学]