Rapid detection of Shigella and Salmonella in rhesus monkeys by loop-mediated isothermal amplification assay  

作　　者：叶莉 吴芳 WANG Yi-jia ZHENG Jun-wen FAN Jun-wen DING Ming SHI Yan-sheng 张小飞 白杰英 

机构地区：[1]Laboratory Animal Center, Academy of Military Medical Sciences, Beijing, 100071, China [2]the Sixth Clinical School, Capital Medical University, Beijing 100029 [3]Capital Normal University, Beijing, 100048 [4]Beijing Institute of Veterinary Drug Control, Beijing 102600

出　　处：《中国实验动物学报》2016年第1期7-13,共7页Acta Laboratorium Animalis Scientia Sinica

基　　金：十二五后勤科研项目(CWS11J092);国家自然科学基金(31272385);十二五重大专项(2011ZXJ09201-031);北京市自然科学基金(5152023)

摘　　要：Objective To establish a loop-mediated isothermal amplification( LAMP) method for detecting diarrhea pathogens( Shigella and Salmonella) in rhesus monkeys and evaluate the application of the LAMP method for detecting bacterial diseases in nonhuman primate laboratory animals. Materials and Methods A total of 205 fecal samples of rhesus monkeys were detected in this LAMP assay. The specificity and sensitivity of LAMP for Shigella and Salmonella were analyzed,and real-time polymerase chain reaction( REAL-TIME PCR) assay was employed as control. Results The LAMP method established here needed only 45 min to complete the reaction at 63℃. Its detection limit was 10 pg / μL and with a high specificity. The positive rate of Shigella and Salmonella was 1. 5% and 6. 3%,respectively. Conclusions Here we have established a fast and simple Shigella and Salmonella LAMP detection method that has strong specificity and high sensitivity and is suitable for rapid detection of bacterial disease in macaques. The development of this rapid detection kit is underway,and it will be helpful to the diarrhea detection.Objective To establish a loop-mediated isothermal amplification（ LAMP） method for detecting diarrhea pathogens（ Shigella and Salmonella） in rhesus monkeys and evaluate the application of the LAMP method for detecting bacterial diseases in nonhuman primate laboratory animals. Materials and Methods A total of 205 fecal samples of rhesus monkeys were detected in this LAMP assay. The specificity and sensitivity of LAMP for Shigella and Salmonella were analyzed,and real-time polymerase chain reaction（ REAL-TIME PCR） assay was employed as control. Results The LAMP method established here needed only 45 min to complete the reaction at 63℃. Its detection limit was 10 pg / μL and with a high specificity. The positive rate of Shigella and Salmonella was 1. 5% and 6. 3%,respectively. Conclusions Here we have established a fast and simple Shigella and Salmonella LAMP detection method that has strong specificity and high sensitivity and is suitable for rapid detection of bacterial disease in macaques. The development of this rapid detection kit is underway,and it will be helpful to the diarrhea detection.

关 键 词：经典条件反射 操作式条件反射 SD大鼠 WISTAR大鼠 学习记忆 

分 类 号：Q95-33[生物学—动物学]