复合刺激创伤后应激障碍模型大鼠重要脑区的病理变化  

Pathological changes in some important brain areas of rats with post-traumatic stress disorder induced by combined stress

作　　者：陈旺[1,2] 李陈成[1,2] 李森[2] 冯东亮[3] 洪有建 南伟[3] 江龙[1,2] 黄显凯[1] 伍亚民[2] 

机构地区：[1]第三军医大学大坪医院野战外科研究所创伤外科,重庆400042 [2]第三军医大学大坪医院野战外科研究所第三研究室,创伤,烧伤与复合伤国家重点实验室,重庆400042 [3]兰州大学第二临床医学院骨科,兰州730030

出　　处：《中国实验动物学报》2016年第1期87-91,共5页Acta Laboratorium Animalis Scientia Sinica

基　　金：全军医药卫生科研基金(08G096)

摘　　要：目的研究复合刺激创伤后应激障碍(PTSD)模型大鼠重要脑区的病理变化。方法成年SD雌性大鼠20只,随机分为正常组、模型组,每组10只。模型组按复合刺激方法建立PTSD大鼠模型,4周后对两组大鼠行高架十字迷宫和Morris水迷宫检测;行为检测结束后对大鼠大脑皮层和海马CA1、CA2、CA3区和齿状回进行HE染色和Nissl染色,观察其病理变化特点。结果正常组大脑皮层和海马各区细胞形态规则、分布均匀,核仁及边界清晰,胞质尼氏体丰富,无明显神经元变性坏死;模型组大脑皮层细胞形态较规则、分布均匀,无明显病理改变;海马CA1、CA3区细胞形态不规则,排列紊乱,细胞间隙增大,细胞数量减少,有较多空泡样细胞病变,CA3区更为明显;CA2区细胞排列较规则,分布较均匀;齿状回可见部分细胞排列疏松、细胞间隙增大、尼氏体数量减少。结论PTSD模型大鼠海马CA1、CA3区和齿状回均有不同程度的病理改变,为探讨PTSD患者的病理机制提供了实验依据。Objective To study the pathological changes in some important brain areas of rats with post-traumatic stress disorder（ PTSD） induced by combined stress. Methods Twenty healthy adult female SD rats were randomly divided into normal group and model group,with 10 rats in each group. The PTSD model was established by combined stress. After4 weeks,the rat behaviors of the two groups were tested by elevated plus maze and Morris water maze. After the test,samples of the cerebral cortex,areas of CA1,CA2,CA3 and hippocampal dentate gyrus were taken for pathological examination using HE and Nissl staining. Results In the normal group,the cell morphology and distribution were regular and the cell nucleoli were clear in the cerebral cortex and hippocampus,cytoplasmic Nissl bodies were abundant,and there were no obvious neuronal degeneration and necrosis. In the model group,the cell morphology and distribution in the CA1 and CA3 areas were irregular,with increased cell gaps,and in addition,there were some pathological changes of physaliferous cells,especially in the CA3 area. The cell morphology and distribution in the CA2 area were regular. In the dentate gyrus,some cells arranged loosely,the cell gap was widened,and the amount of Nissl bodies was decreased. Conclusions There are some pathological changes in the hypocampal CA1,CA3 and dentate gyrus in rat models of post-traumatic stress disorder,and they will provide an experimental basis for studying the pathological mechanism of PTSD patients.

关 键 词：创伤后应激障碍 大脑皮层 海马 病理学 大鼠 

分 类 号：Q95-33[生物学—动物学]