两品系豚鼠屈光状态和眼球径的发展和比较  

Comparison of the refractive development and ocular growth between two strains of guinea pigs

作　　者：卫振[1] 张森[2] 蒋丽琴[2] 陈雁虹[1] 吴凯[1] 谢敏[1] 刘迪文[1] 谢强敏[1] 

机构地区：[1]浙江大学实验动物中心,杭州310058 [2]温州医科大学,温州325035

出　　处：《中国实验动物学报》2016年第1期92-96,共5页Acta Laboratorium Animalis Scientia Sinica

基　　金：浙江省公益性技术应用研究计划(2012C37085);浙江省医药卫生科技计划(2014KYB074)

摘　　要：目的了解断奶后Zmu-1：DHP和DHP两个品系豚鼠屈光状态和眼球径的发展规律。方法首先进行3周龄两品系豚鼠屈光检测,然后选择Zmu-1：DHP品系豚鼠12只,DHP品系8只,按品系分成两组。在4~12周龄,每隔一周检测屈光度、眼轴长和角膜曲率等参数。结果 3周龄,327只Zmu-1：DHP品系豚鼠,屈光度为-5.19±3.92 D,近视率达85.1%;而117只DHP品系豚鼠屈光度为2.13±4.40 D,近视率仅为22.6%。4~12周龄,Zmu-1：DHP品系豚鼠近视不断加深,屈光参差〉1.5 D;DHP品系豚鼠屈光发育稳定。t-检验,Zmu-1：DHP品系豚鼠眼轴长度、玻璃体腔深度显著高于DHP品系,视力低于DHP品系。多因素方差分析,Zmu-1：DHP品系豚鼠散光随时间变化显著（P〈0.01）,而DHP品系不显著（P=0.07）。相关性分析,Zmu-1：DHP品系豚鼠屈光状态与眼轴长相关系数为-0.79（P〈0.01）、与玻璃体腔深度相关系数为-0.75（P〈0.01）;而DHP品系相应相关系数为-0.19（P=0.09）和-0.34（P〈0.01）。结论 Zmu-1：DHP品系豚鼠近视高发,正视化过程难以完成,其近视与眼轴长度和玻璃体腔深度高度相关。Object To compare the refractive development and axial eye growth between Zmu-1： DHP and DHP guinea pig strains after weaning. Methods After screening of the refractive status of two strains of guinea pigs at 3 weeks of age,twelve Zmu-1： DHP guinea pigs and eight DHP guinea pigs were chosen. Each group underwent a series of ocular measurements at five time-points（ 4,6,8,10 and 12 weeks）,including refraction,axial length,corneal curvature and other ocular parameters. Results The refraction of 3-week old Zmu-1： DHP strain（ n = 327） was- 5. 19 ± 3. 92 D with a myopic rate of 85. 1%. While the refraction of DHP strain guinea pigs（ n = 117） was 2. 13 ± 4. 40 D with a myopic rate of22. 6%. From 4 to 12 weeks of age,the guinea pigs of Zmu-1： DHP strain developed toward more myopic,their anisometropia was larger than 1. 5 D. The DHP strain had a stable refractive state. The Zmu-1： DHP guinea pig strain had larger axial length and vitreous thickness than strain DHP（ t-test： P〈0. 01）,and the DHP guinea pig strain had better vision（ P〈0. 01）. Astigmatism of Zmu-1： DHP strain guinea pigs changed significantly from 4 to 12 weeks（ ANOVA： P〈0. 01）,while DHP strain did not（ ANOVA： P = 0. 07）. In the Zmu-1： DHP strain,the refractive status had significant correlation with axial length（ r =- 0. 785,P〈0. 01） and vitreous chamber depth（ r =- 0. 754,P〈0. 01）. In the DHP strain,the correlation coefficients were- 0. 19（ P = 0. 089） and- 0. 34（ P〈0. 01）. Conclusions Zmu-1： DHP strain guinea pigs have a higher rate of spontaneous myopia than DHP strain. The emmetropization is disrupted. This myopia is highly correlated with axial length and vitreous chamber depth.

关 键 词：豚鼠 近视 屈光 眼轴长 角膜曲率 

分 类 号：Q95-33[生物学—动物学]