不同炮制方法对延胡索中延胡索乙素含量的影响  

Influence of Different Processing Methods on Tetrahydropalmatine in Corydalis Rhizoma

作　　者：肖辉[1] 

机构地区：[1]湖南中医药大学第一附属医院药学部,湖南长沙410007

出　　处：《中国药业》2016年第4期29-31,共3页China Pharmaceuticals

摘　　要：目的比较醋煮、醋烘、醋炙、酒炙等炮制方法对延胡索有效成分含量的影响,为优化延胡索炮制方法提供参考。方法采用醋煮、醋烘、醋炙、酒炙等炮制方法对延胡索生品进行炮制,通过高效液相色谱法测定延胡索乙素含量。结果延胡索乙素含量在0.10-1.35μg范围内与峰面积线性关系良好,平均回收率为84.05%,RSD为1.94%（n=6）;醋烘、醋炙、酒炙延胡索炮制品含量有所上升,差异有统计学意义（t=3.265-6.342,P〈0.05）。结论高效液相色谱法检测延胡索乙素具有线性关系良好、精密度稳定、可重复检测的优点,醋烘、醋炙、酒炙等炮制方法能增加延胡索有效成分含量。Objective To compare the influence of vinegar boiling,vinegar baking,vinegar stir-frying,wine stir-frying on the active ingredients of corydalis rhizoma.Methods Corydalis rhizoma was processed by vinegar boiling,vinegar baking,vinegar stir-frying;wine stir-frying;HPLC method was used to determine the content of tetrahydropalmatine.Results The content of tetrahydropalmatine showed good linear relationship wasa the peak area in the range of 0.10-1.35 μg;the average recovery rate was 84.05%,RSD was1.94%（n=6）;the main content of tetrahydropalmatine improved after vinegar boiling,vinegar baking,vinegar stir-frying,and wine stir-frying,and there were statistically difference in the 4 methods（t=3.265-6.342,P0.05）.Conclusion HPLC method for detection of tetrahydropalmatine has a good linear relationship /Stable precision,repeatable testing;the processing methods of vinegar baking,vinegar stir-frying and wine stir-frying can increase the effective composition content in corydalis rhizoma.

关 键 词：延胡索 延胡索乙素 炮制 高效液相色谱法 

分 类 号：R283[医药卫生—中药学] R284.1[医药卫生—中医学]