羟基红花黄色素A对淀粉样蛋白诱导的氧化应激和学习记忆功能损伤的影响  

Effects of hydroxysafflor yellow A on β-amyloid-induced oxidative stress and dysfunction of learning and memory in rats

作　　者：王颖[1] 鲁雅琴[1] 蒋珍秀[1] 雒彧[2] 

机构地区：[1]兰州大学第一医院神经内科 [2]兰州大学基础医学院病理生理学研究所

出　　处：《中国临床药理学与治疗学》2016年第1期60-63,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：兰州大学中央高校基本科研业务费专项资金资助项目(lzujbky-2014-140)

摘　　要：目的:研究羟基红花黄色素A(HSYA)对β淀粉样蛋白(Aβ)诱导的氧化应激和学习记忆功能损伤的影响。方法:通过侧脑室注射Aβ建立学习记忆功能损伤的动物模型,术后通过腹腔注射HSYA进行干预,采用Morris水迷宫实验来检测学习记忆功能的改变,测定大鼠脑组织中丙二醛(MDA)、超氧化物歧化酶(SOD)和还原型谷胱甘肽(GSH)的含量以及炎症因子IL-6和TNF-α的水平。结果:HSYA能够改善由Aβ诱导的学习记忆功能损伤,降低脑组织海马中MDA的含量,增加SOD和GSH的含量;降低脑组织海马中IL-6和TNF-α的水平。结论:HSYA能抑制由Aβ诱导的脑组织中氧化应激水平和炎症反应,改善由Aβ诱导的学习记忆功能损伤。AIM： To study the effects of hydroxysafflor yellow A（ HSYA） on β-amyloid-induced oxidative stress and dysfunction of learning and memory.METHODS： Rats were randomly divided into four groups： control group,sham operation group,NS（ normal saline） group and HSYA group.The animal model for dysfunction of learning and memory was established by intracerebroventricular injection of β-amyloid in NS and HSYA groups.The HSYA group was received 6 mg/kg per day HSYA intraperitoneally for 14 days after intracerebroventricular injection.The other three groups were received the same amount normal saline for 14 days after operation.The Morris water maze was used to evaluate the function of spacial learning and memory.The levels of MDA,SOD,GSH,IL-6 and TNFin rats＇ hippocampus were measured after the Morris water maze test.RESULTS： HSYA reversed β-amyloid-induced cognitive impairment.HSYA reduced the levels of MDA,IL-6,TNF-α and increased the levels of SOD,GSH in rats ＇ hippocampus.CONCLUSION： HSYA can attenuate β-amyloid-induced oxidative stress reaction and reverse β-amyloid-induced cognitive impairment in brain of rats.

关 键 词：羟基红花黄色素A Β淀粉样蛋白 学习记忆 氧化应激 

分 类 号：R965.2[医药卫生—药理学]