基于激光共聚焦显微镜λ扫描检测蒿草切片荧光光谱的研究  

Study on the fluorescence spectrum of wormwood slice with laser scanning confocal microscope

作　　者：吴伟全[1] 李元歌[2] 王思捷[1] 杨腾[1] 吴平[1] 

机构地区：[1]广东医学院临床医学研究中心,广东湛江524001 [2]广东医学院附属医院放射科,广东湛江524001

出　　处：《实验与检验医学》2016年第1期21-23,共3页Experimental and Laboratory Medicine

基　　金：广东医学院科研基金项目(M2014044)

摘　　要：目的研究激光扫描共聚焦显微镜λ扫描检测蒿草切片的荧光光谱,为基于共聚焦显微镜获得生物医学样本的荧光光谱提供理论和实验依据。方法运用普通荧光显微镜和激光扫描共聚焦显微镜分别观察荧光染色的蒿草切片,实验分为三组:A组用普通荧光显微镜观察并拍照;B组用激光扫描共聚焦显微镜检测荧光光谱;C组用激光扫描共聚焦显微镜扫描荧光图像。结果荧光显微镜可以观察到蒿草切片的荧光图像,但不能准确获取其相应荧光激发和发射波长,而且会发生串色干扰现象。激光扫描共聚焦显微镜能对蒿草切片进行荧光光谱扫描,排除了干扰波长,获取其荧光激发和发射波长,得到高清晰度的荧光图像。结论与普通荧光显微镜比较,激光扫描共聚焦显微镜有能准确获取样本荧光光谱,得到高清晰度荧光图像的优点。Objective To study the fluorescence spectrum of wormwood slice with laser confocal microscope,and provide the theoretical and experimental basis for obtaining the fluorescence spectrum of biomedical samples. Methods The fluorescence microscope and laser scanning confocal microscope were used to observe fluorescence of wormwood slice. The experiment included three groups：group A,using fluorescence microscope to take photos;group B,using laser scanning confocal microscope to detect fluorescence spectrum;group C,using laser scanning confocal microscope to obtain fluorescence image. Results The fluorescence microscope could observe the fluorescence image of wormwood slice,but could not accurately obtain the corresponding fluorescence excitation and emission wavelength. The channeling color interference phenomenon was occurred. The laser scanning confocal microscope could scan the fluorescence spectrum of wormwood slice,exclude the interference wavelength,obtain the fluorescence excitation and emission wavelength accurately and gather fluorescent image with high-resolution. Conclusion Compared with the fluorescence microscope,the laser scanning confocal microscope can accurately obtain the fluorescence spectrum and high-resolution fluorescence image of biomedical samples.

关 键 词：激光共聚焦显微镜 蒿草切片 荧光 光谱分析 

分 类 号：R318.51[医药卫生—生物医学工程]