SD大鼠妊娠期高血糖对子代糖代谢、脂质代谢和动脉粥样硬化发病的影响  

Effect of severe maternal hyperglycemia on sugar and fat metabolism and atherosclerosis in Sprague-Dawley rat offspring

作　　者：黄婕[1] 宋治远[2] 黄骥[1] 

机构地区：[1]武警重庆市总队医院心血管内科,重庆400061 [2]第三军医大学西南医院心内科,重庆400038

出　　处：《中国循证心血管医学杂志》2016年第2期220-223,共4页Chinese Journal of Evidence-Based Cardiovascular Medicine

摘　　要：目的通过链脲佐菌素(streptozocin,STZ)腹腔注射诱发雌性SD大鼠妊娠期高血糖,研究妊娠期高血糖对雄性子代成年后糖代谢、脂代谢和动脉粥样硬化发病的影响。方法妊娠期糖尿病造模实验分为两组:母代对照组和糖尿病组,均为8周龄健康成年雌性SD大鼠(体重200 g^250 g),分别与健康雄性SD大鼠交配。观察到阴栓后,糖尿病组母鼠腹腔注射40 mg/kg STZ溶液,对照组母鼠柠檬酸-柠檬酸钠缓冲液腹腔注射。两组母鼠产仔后取其雄性子代正常喂养,分别记为对照子代组和糖尿病子代组。子代25周龄时采用糖耐量实验和胰岛素耐量实验检测两组子代糖代谢情况。采用血生化分析仪检测子代血脂;采用PCR检测心血管动脉粥样硬化标志性基因E-selectin、VCAM-1、ICAM-1、ICAM-2。结果妊娠期给予母鼠40 mg/kg STZ注射能导致妊娠期高血糖;糖尿病子代组大鼠比子代对照组糖耐量和胰岛素耐量均出现受损趋势(P<0.05);糖尿病子代组E-selectin、VCAM-1、ICAM-1表达比对照子代组升高(P<0.05)。结论母亲妊娠期糖尿病可能是诱发子代成年期糖代谢异常、脂代谢紊乱和动脉粥样硬化的一个高风险因素。Objective By intraperitoneal injection of streptozocin（STZ）on maternal, to investigate the effect of maternal hyperglycemia on sugar and fat metabolism and atherosclerosis in SD（Sprague-Dawley） rat offspring. Methods SD rats（8-week-old, weighing 200~250 g） were used in this study. The maternal control group, diabetes group were established according to the experimental requirements. Maternal diabetes group was induced by a 40 mg/kg intraperitoneal injection of STZ on the first day of gestation. Maternal control group was injected with citrate buffer. The offspring were feeding normally and marked as offspring control group and offspring diabetes group. Glucose and insulin tolerance tests（GTT and ITT） were performed; and serum levels of lipids were measured in offspring groups. PCR was performed to analyze m RNAs expression of E-selectin, VCAM-1, ICAM-1 and ICAM-2 in these groups. Results Given 40 mg / kg STZ to pregnant rats can lead to gestational diabetes. Compared with offspring control group, offspring diabetes group showed more severe impairment in GTT and ITT,had higher level of blood lipid and m RNAs expression of E-selectin, VCAM-1, ICAM-1. Conclusion Severe maternal hyperglycemia may be a high-risk factor of sugar and fat metabolism and atherosclerosis in offspring.

关 键 词：妊娠期糖尿病 大鼠 子代 代谢 动脉粥样硬化 

分 类 号：R587.1[医药卫生—内分泌]