新型氯化锂复合磷酸钙骨水泥的理化性能及成骨特性  

Novel lithium chloride/calcium phosphate cement: physicochemical and osteogenic properties

作　　者：李理[1] 李百川[1] 王仁崇[1] 李兵[1] 

机构地区：[1]广西医科大学第四附属医院/柳州市工人医院,广西壮族自治区柳州市545005

出　　处：《中国组织工程研究》2016年第3期307-313,共7页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金(81260273);广西壮族自治区自然科学基金(2013GXNSFAA019269)~~

摘　　要：背景:氯化锂是目前广泛应用的GSK-3β无机离子抑制剂,可通过与GSK-3β结合,激活经典Wnt/β-catenin通道进而促进人骨髓间充质干细胞、成骨细胞的增殖,促进骨细胞修复。目的:观察新型氯化锂复合磷酸钙骨水泥(Lithium chloride/calcium phosphate cement,Li/CPC)的理化性质,探索其诱导成骨的生物学性能。方法:以磷酸钙骨水泥为空白对照组,不同锂含量的Li/CPC为实验组,检测各组骨水泥的凝固时间、抗压强度,扫描电镜观察材料表面微观结构;将各组骨水泥与MC3T3-E1细胞在体外共培养,用扫描电子显微镜观察细胞在骨水泥表面的生长及黏附形态,MTT法检测骨水泥浸提液对细胞体外增殖的影响,碱性磷酸酶试剂盒检测骨水泥浸提液对细胞碱性磷酸酶活性的影响。结果与结论:Li Cl的加入未改变磷酸钙骨水泥的理化性质,各组间初凝时间与终凝时间及抗压强度未见明显差异,各组电镜下微观形貌也未见明显差异;MC3T3-E1细胞能够很好地在材料表面黏附生长,MTT法检测结果表明Li/CPC较磷酸钙骨水泥更能够促进骨细胞的体外增殖,而碱性磷酸酶测试结果则表明,MC3T3-E1细胞在Li/CPC有更高的碱性磷酸酶活性。Li/CPC保持良好理化性质的同时,释放出锂离子作用于外周发挥其促进成骨作用。BACKGROUND： Lithium chloride is a widely used inorganic ion inhibitor of glycogen synthase kinase-3β, and it can be combined with glycogen synthase kinase-3β to activate the classical Wnt/β-catenin pathway, thereby promoting human bone marrow mesenchymal stem cells and osteoblasts proliferation and accelerating bone repair. OBJECTIVE： To observe the physicochemical properties of novel lithium chloride/calcium phosphate cement, and to explore its osteoinductive biological property. METHODS： Calcium phosphate cement served as control group, and lithium chloride/calcium phosphate cement containing different lithium content as experimental groups. The setting time and compressive strength of bone cement in each group were detected, and the microstructure of the material surface observed under scanning electron microscopy. Bone cement and MC3T3-E1 cells were co-cultured in vitro, and the growth and adhesion morphology of MC3T3-E1 cells on the surface of bone cement were observed under the scanning electron microscope. Effect of bone cement extracts on cell proliferation was determined through MTT assay, and alkaline phosphatase kit used for determining alkaline phosphatase activity. RESULTS AND CONCLUSION： Lithium chloride/calcium phosphate cement had the same physicochemical properties to the calcium phosphate cement. Initial and final setting time, compressive strength and morphology of bone cement had no significant differences among groups. MC3T3-E1 cells grew and adhered well on the material surface. Results of MTT assay showed that compared with the calcium phosphate cement, the lithium chloride/calcium phosphate cement was better to improve osteoblast proliferation in vitro. In addition, the alkaline phosphatase activity in MC3T3-E1 cells was higher in experimental groups than the control group. These findings indicate that lithium chloride/calcium phosphate cement can maintain good physicochemical properties, and release lithium ions to promote bone formation.

关 键 词：氯化锂 磷酸钙类 骨细胞 组织工程 生物材料 骨生物材料 磷酸钙骨水泥 氯化锂/磷酸钙复合骨水泥 MC3T3-E1细胞 抗压强度 细胞增殖 碱性磷酸酶 成骨 国家自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]