树脂水门汀口腔黏结材料SuperBond C&B的体外细胞毒性  

In vitro cytotoxicity of Super Bond C&B, a resin cement for dental bonding

作　　者：江先敏 赵曦[1] 

机构地区：[1]德阳市人民医院口腔科,四川省德阳市618000

出　　处：《中国组织工程研究》2016年第3期341-345,共5页Chinese Journal of Tissue Engineering Research

摘　　要：背景:口腔黏结材料长期与牙体组织、牙龈组织及口腔中唾液相接触,所以必须具备生物安全性、低细胞毒性或无细胞毒性等特点,保证对口腔黏膜无刺激,与人体组织接触不会发生毒性及过敏反应等。目的:观察口腔黏结材料Super Bond C&B超级粘接剂的体外细胞毒性。方法:取对数期人牙周膜成纤维细胞,以1.0×10~8 L^(-1)的细胞浓度接种在96孔板上,100μL/孔,常规培养24 h弃上清,分组培养:实验组加入90μL DMEM培养液+10μL Super Bond C&B超级粘接剂浸提液,阴性对照组加入100μL培养液,阳性对照组加入100μL苯酚溶液。培养72 h后,利用锥虫蓝排斥实验计算各组细胞存活率。结果与结论:实验组、阴性对照组细胞存活率均高于阳性对照组(P<0.05),实验组与阴性对照组细胞存活率比较差异无显著性意义(P>0.05)。结果表明Super Bond C&B超级粘接剂无细胞毒性。Because of long-term contact with tooth tissues, gingival tissues and saliva in the mouth, oral bonding materials must have the characteristics of biological safety and low or no cytotoxicity, so as to ensure no irritation response to the oral mucous and no toxicity and allergic reactions when contacting with human tissues. OBJECTIVE： To observe the in vitro cytotoxicity of an oral bonding material, SuperBond C＆B. METHODS： The logarithmic human periodontal ligament fibroblasts were obtained and inoculated in 96-well plates with a cell concentration of 1.0×10^8/L 100 IJIJhole. Supernatant was removed after 24 hours of routine culture, and the specimens were grouped and cultured. 90 IJL DMEM culture liquid＋10 μL SuperBond C＆B were added in the experimental group. 100 μL medium was added in the negative control group. 100 tJL phenol solution was added in the positive control group. After 72 hours of culture, the cell survival rate in each group was calculated by trypan blue exclusion test.RESULTS AND CONCLUSION： The cell survival rates of the experimental group and the negative control group were higher than that of the positive control group （P 〈 0.05）, and there was no significant difference between the experimental group and the negative control group （P 〉 0.05）. These results show that SuperBond C＆B has no cytotoxicity.

关 键 词：牙周膜 成纤维细胞 牙科粘固剂 组织工程 生物材料 口腔生物材料 口腔黏结材料 树脂水门汀 人牙周膜成纤维细胞 锥虫蓝排斥实验 细胞毒性 

分 类 号：R318[医药卫生—生物医学工程]