构建双足类大型鸟类鸸鹋股骨头坏死模型  

Establishing a large double-foot bird emu model of femoral head necrosis

作　　者：曲春涛 

机构地区：[1]天津市红桥医院,天津市300131

出　　处：《中国组织工程研究》2016年第5期611-615,共5页Chinese Journal of Tissue Engineering Research

摘　　要：背景:虽然对股骨头坏死动物模型的建立已经进行了许多研究,然而到目前为止仍难以模拟出理想的股骨头坏死的动物模型,尤其是股骨头塌陷模型。目的:建立双足类大型鸟类动物鸸鹋股骨头坏死模型,用于更好模拟人类股骨头坏死的治疗研究。方法:成年鸸鹋30只,建立液氮冷冻并射频加热交替损伤股骨头坏死模型。冷冻3个循环,采用局部液氮冷冻和射频加热交替的方法,进行闭合回路的液氮冷冻损伤和射频加热交替的损伤,造模后第6,12,16周进行大体观察、X射线、组织学检查和MRI检查,评价鸸鹋股骨头缺血坏死模型建模效果。结果与结论:经过液氮冷热交替法进行造模的鸸鹋于12-18周逐步出现股骨头坏死征象和影像学表现,通过X射线,组织学检查和MRI检查可见其股骨头坏死过程符合人类股骨头坏死病变的病理过程,且模型具有良好的可重复性。结果提示,采用液氮冷热交替法成功建立了鸸鹋塌陷性股骨头骨坏死模型,具有较好的塌陷率,可以用于骨坏死的机制研究和治疗评价。BACKGROUND： There are many studies on the establishment of animal models of femoral head necrosis, but it is difficult to simulate an ideal animal model of femoral head necrosis, especially collapse models. OBJECTIVE： To establish femoral head necrosis model of double-foot large bird emu so as to simulate femoral head necrosis in human. METHODS： A total of 30 adult emus were frozen in liquid nitrogen and received radiofrequency heating alternately to simulate femoral head necrosis models. After 3 cycles of freezing, local frozen in liquid nitrogen and method of radiofrequency heating was alternated for closed loop liquid nitrogen freezing injury and radiofrequency heating injury. At 6, 12 and 16 weeks after model establishment, gross observation, X-ray, histology examination and MRI were conducted. Effects of model induction of avascular necrosis of the femoral head were evaluated. RESULTS AND CONCLUSION： After liquid nitrogen cold-hot alternating method, emu at 12-18 weeks gradually suffered from femoral head necrosis signs and imaging findings. X-ray films, histological examination and MRI examination showed the avascular necrosis of femoral head was consistent with the pathological process of human femoral head necrosis, and the model had good repeatability. These results indicate that the use of liquid nitrogen cold-hot alternating method has successfully established the model of femoral head necrosis, with a good collapse rate, and can be used for mechanism research and treatment evaluation of osteonecrosis.

关 键 词：鸸鹋科 股骨头坏死 模型 动物 组织工程 实验动物 骨软骨损伤与修复动物模型 鸸鹋 液氮冷热交替法 重复性 动物模型 

分 类 号：R318[医药卫生—生物医学工程]