SD大鼠脊髓损伤后微环境的模拟实验  

Simulation of microenviroment after spinal cord injury in Sprague-Dawley rats

作　　者：贾叙锋[1,2] 龙苗[1] 戢勇[1] 黄光平[1] 周玉[1] 张方德[1] 冯大雄[2] 

机构地区：[1]简阳市人民医院骨科,四川省简阳市641400 [2]泸州医学院附属医院脊柱外科,四川省泸州市646000

出　　处：《中国组织工程研究》2016年第5期628-634,共7页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金资助(30872602)~~

摘　　要：背景:通过制作SD大鼠的轻、中、重度脊髓损伤模型,使之符合基础实验研究的脊髓损伤类型,可进一步了解SD大鼠脊髓损伤后的微环境变化,为临床治疗提供帮助。目的:模拟SD大鼠脊髓损伤后微环境,观察不同致伤势能的Allen’s模型和脊髓全切模型SD大鼠脊髓损伤后不同时间点的神经功能、病理以及运动诱发电位的变化。方法:选择125只成年雌性健康SD大鼠,随机分为假手术组、打击势能100 gcf组(20 g×5 cm)、打击势能200 gcf组(20 g×10 cm)、打击势能300 gcf组(20 g×15 cm)、脊髓全切组,每组25只,在制模成功后第1,5,7,14,28天采用BBB后肢运动功能评分、运动诱发电位监测、病理切片鉴定,观察各组大鼠脊髓损伤程度。结果与结论:1整个实验中SD大鼠死亡24只,脊髓全切组的死亡率和并发症最高,每组的BBB评分均降低,随着时间延长BBB评分逐渐增高。各手术组与假手术组在对应时间点比较,差异有显著性意义(P<0.05),打击势能300 gcf组和脊髓全切组在对应时间点比较,差异无显著性意义(P>0.05)。2各手术组在伤后1 d病灶处出现炎性细胞浸润,神经元细胞肿胀明显,随着时间推移,神经细胞减少;伤后28 d发现大量星形胶质细胞增生,瘢痕及脊髓空洞形成,打击势能300 gcf组、脊髓全切组明显重于打击势能100 gcf组、打击势能200 gcf组。3各手术组与假手术组的波幅及潜伏期在对应时间点比较,差异有显著性意义(P<0.05);打击势能300 gcf组和脊髓全切组在对应时间点比较,差异无显著性意义(P>0.05)。结果表明致伤势能为20 g×5 cm,20 g×10 cm,20 g×15 cm,可模拟SD大鼠轻、中、重度脊髓损伤模型的微环境;不同势能的改良Allen’s模型较脊髓全切模型并发症低,更符合基础研究。BACKGROUND： We built Sprague-Dawley rat models with mild, moderate, and severe spinal cord injuries to accord with the spinal cord injury types for basic empirical study, and consequently to further understand the microenvironmental change in Sprague-Dawley rats with spinal cord injury, and to provide help for clinical treatment. OBJECTIVE： To observe the changes in nerve function, pathological manifestation and motor sensory evoked potential in Allen＇s models and Sprague-Dawley rats with complete spinal cord transection at different time points after spinal cord injury by simulating the microenviroment in Sprague-Dawley rats. METHODS： A total of 125 healthy adult female Sprague-Dawley rats were selected and randomly divided into group sham operation group, 100 gcf hit potential group（20 g×5 cm）, 200 gcf hit potential（20 g×10 cm）, 300 gcf hit potential group（20 g×15 cm）, and spinal cord complete transection group with 25 rats in each group. At 1, 5, 7, 14 and 28 days after model establishment, the degree of spinal cord injury was identified by the BBB scores of motion function, motor evoked potential, and pathological section. RESULTS AND CONCLUSION：（1） Totally 24 Sprague-Dawley rats died in the experiment. The death rate and the rate of complications were highest in the spinal cord complete transection group. The BBB score of each group was decreased. The BBB scores in every group increased as time went on. There were significant differences between each surgery group and the sham operation group at corresponding time points（P〈0.05）. No significant difference was found between the 300 gcf hit potential group and the spinal cord complete transection group at corresponding time points（P〉0.05）.（2） In each surgery group, the infiltration of inflammatory cells and obvious swelling of neurons were visible at 1 day after injury. Neural cells reduced with time prolonged. At 28 days after injury, a large number of astrocytes proliferated, scar and spinal cord cavity formed. Ab

关 键 词：脊髓损伤 模型 动物 大鼠 Sprague-Dawley 组织工程 实验动物 神经损伤与修复动物模型 Allen’s模型 脊髓全切模型 SD大鼠 微环境 国家自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]