人工虫草复合液对无心跳模型大鼠离体肾的低温保护作用  

Protective effect at low temperature of artificial cordyceps compound on isolated kidney from non-heart-beating rat models

作　　者：戴晨[1] 王顺[1] 徐加波[2] 刘健[1] 

机构地区：[1]新疆医科大学第一附属医院肾病科,新疆维吾尔自治区乌鲁木齐市830000 [2]新疆工程学院,新疆维吾尔自治区乌鲁木齐市830000

出　　处：《中国组织工程研究》2016年第5期664-670,共7页Chinese Journal of Tissue Engineering Research

基　　金：新疆维吾尔自治区自然科学基金项目(2011211A058);国家自然科学基金项目(青年基金)(11301451)~~

摘　　要：背景:无心跳供体已成为近年来最有潜力的移植供体来源,为进一步改进供体保存液,在常规保存液高渗枸橼酸盐腺嘌呤液的基础上加入人工虫草制备人工虫草复合液,希望通过其抗氧化作用,减少供体损伤,提高供体质量。目的:探讨人工虫草复合液对无心跳大鼠离体肾的保存作用。方法:将39只SD大鼠毁损延髓,不进行体外呼吸循环支持,造成脑死亡诱导的无心跳模型后,随机等分为3组,人工虫草复合液组(n=15)、高渗枸橼酸盐腺嘌呤液组(n=15)和生理盐水组(n=9),分别用人工虫草复合液、高渗枸橼酸盐腺嘌呤液、生理盐水对离体肾脏进行4℃低温保存。结果与结论:与高渗枸橼酸盐腺嘌呤液组相比,人工虫草复合液组肾组织保存6和12 h后,形态损伤较轻,凋亡指数、丙二醛含量、Bax及Caspase-3表达水平下降,超氧化物歧化酶活力及Bcl-2表达水平上升;而保存24 h后,形态损伤程度接近,丙二醛含量下降,超氧化物歧化酶活力上升。提示人工虫草复合液对无心跳大鼠离体肾的抗氧化、抑制细胞凋亡方面保存效果优于高渗枸橼酸盐腺嘌呤液。BACKGROUND： Non-heart beating donors have become the most promising source of donor in recent years. Tofurther improve donor preservation solution, on the basis of conventional storage solution hypertonic citrate adenine solution, artificial cordyceps was added to prepare artificial cordyceps compound solution. It is hoped to reduce donor injury and to elevate donor quality by antioxidation. OBJECTIVE： To investigate the preservation effect of artificial cordyceps compound solution on isolated renal from non-heart-beating rats. METHODS： Medullas of 39 Sprague-Dawley rats were injured. Without in vitro respiratory and circulatory supports, non-heart-beating models were established and randomly divided into three groups： artificial cordyceps compound group（n=15）, hypertonic citrate adenine solution group（n=15） and physiological saline group（n=9）. The kidneys of rats in different groups were stored in artificial cordyceps compound solution, hypertonic citrate adenine solution and physiological saline at 4 ℃. RESULTS AND CONCLUSION： Compared with the hypertonic citrate adenine solution group, after 6 and 12 hours of preservation, morphological injury was mild, apoptotic index, malondialdehyde, Bax and Caspase-3 expression levels decreased, superoxide dismutase activity and Bcl-2 expression increased; after 24 hours, the degree of injury was similar, malondialdehyde content decreased, and superoxide dismutase activity increased in the artificial cordyceps compound group. These findings indicated that the preservation effect of artificial cordyceps compound on antioxidation and inhibition of apoptosis was better than that of hypertonic citrate adenine solution in non-hearting beating rats.

关 键 词：冬虫夏草属 肾移植 器官保存 低温保存 实验动物 移植动物模型 无心跳供体 肾脏保存液 缺血再灌注损伤 抗氧化 热缺血 人工虫草 细胞凋亡 国家自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]