祛浊化痰中药干预食饵性动脉粥样硬化模型兔血脂及相关炎性因子的变化  

Dispelling turbid phlegm herb affects the changes in serum lipid and related inflammatory factors in a rabbit model of dietary atherosclerosis

作　　者：宋筱靓[1] 王帅[1] 王凤荣[1] 

机构地区：[1]辽宁中医药大学附属医院,辽宁省沈阳市110032

出　　处：《中国组织工程研究》2016年第5期677-682,共6页Chinese Journal of Tissue Engineering Research

基　　金：辽宁省卫生计生委项目-动脉粥样硬化临床前期病变中医诊疗应用能力建设项目(LNCC-C01-2015)~~

摘　　要：背景:多项动物实验和临床观察证明,祛浊化痰中药对血清脂质、脂蛋白和肝脂质有较好的调节作用。目的:观察祛浊化痰中药对食饵性动脉粥样硬化兔单核细胞趋化因子1、C-反应蛋白及血脂的影响。方法:将50只新西兰大白兔随机分为5组,空白对照组给予基础饲料喂养,同时灌胃给予生理盐水10 mL/(kg·d),连续10周;模型组给予高脂饲料喂养制备动脉粥样硬化模型,同时灌胃给予生理盐水10 mL/(kg·d),连续10周;祛浊化痰预防组给予高脂饲料喂养制备动脉粥样硬化模型,同时灌胃给予祛浊化痰中药10 mL/(kg·d),连续10周;血脂康组给予高脂饲料喂养制备动脉粥样硬化模型,同时一次性灌胃给予血脂康10 mL/(kg·d),连续10周;祛浊化痰治疗组给予高脂饲料喂养10周制备动脉粥样硬化模型,同时灌胃生理盐水10 mL/(kg·d),连续6周,此后改为灌胃给予祛浊化痰中药10 mL/(kg·d),连续4周。第10周末,检测兔血脂、C-反应蛋白、单核细胞趋化因子1 mRNA表达水平,以及取主动脉进行病理观察。结果与结论:血脂康、祛浊化痰中药可通过降低血清总胆固醇、三酰甘油、低密度脂蛋白胆固醇、高密度脂蛋白胆固醇、C-反应蛋白及单核细胞趋化因子1 mRNA表达水平,明显抑制动脉粥样硬化改变,其中祛浊化痰中药预防性应用的效果好于治疗性应用。BACKGROUND： Many animal experiments and clinical observation proved that dispelling turbid phlegm herb has good adjustment function on serum lipid, lipoprotein and liver lipid. OBJECTIVE： To observe the effects of dispelling turbid phlegm herb on monocyte chemotactic factor-I, C-reactive protein and serum lipids in rabbits with dietary atherosclerosis. METHODS： A total of 50 New Zealand white rabbits were randomly divided into five groups. Rabbits in the blank control group were fed with basic feed, and simultaneously intragastrically administrated physiological saline 10 mL/kg per day, for 10 consecutive weeks. Rabbits in the model group were given high-fat diet to prepare atherosclerosis models, and simultaneously intragastrically administrated physiological saline 10 mL/kg per day, for 10 consecutive weeks. Rabbits in the phlegm turbidity treatment group were given high-fat diet to prepare atherosclerosis models, and simultaneously intragastrically administrated dispelling turbid phlegm herb 10 mL/kg per day, for 10 consecutive weeks. Rabbits in the Xuezhikang group were given high-fat diet to prepare atherosclerosis models, and simultaneously administrated Xuezhikang 10 mL/kg per day, for 10 consecutive days. Rabbits in the phlegm turbidity treatment group were given high-fat diet for 10 weeks to prepare atherosclerosis models, and intragastrically administered physiological saline 10 mL/kg per day for 6 weeks, and then given dispelling turbid phlegm herb 10 mL/kg per day for 4 weeks. At 10 weeks, serum lipid, C-reactive protein, and monocyte chemokine 1 mRNA expressions were detected, and pathological observation of the aorta was performed. RESULTS AND CONCLUSION： Xuezhikang and dispelling turbid phlegm herb could decrease serum total cholesterol, triglyceride, low density lipoprotein cholesterol, high density lipid protein cholesterol, C-reactive protein and monocyte chemokine 1 mRNA expression level, and apparently inhibited atherosclerotic changes. The preventive effect of dispelling turbid phlegm

关 键 词：中草药 模型 动物 动脉粥样硬化 组织工程 实验动物 心肺损伤与修复动物模型 祛浊化痰 中药 炎性因子 血脂 

分 类 号：R318[医药卫生—生物医学工程]