Notch1和Bmi-1蛋白在肺癌组织中的表达及意义  

Expression and significance of Notch1 and Bmi-1 protein in lung cancer tissue

作　　者：蔡华荣[1] 江跃全[1] 

机构地区：[1]重庆市肿瘤研究所胸外科,重庆市400030

出　　处：《中国组织工程研究》2016年第5期683-687,共5页Chinese Journal of Tissue Engineering Research

摘　　要：背景:有研究发现,Notch通路与Bmi-1基因均有调节干细胞自我更新的能力,二者功能障碍可能与肿瘤的发生有很大的关系。目的:探讨Notch1和Bmi-1蛋白在肺癌组织中的表达水平及其临床意义。方法:获取87例肺癌患者术后肺癌组织(肺癌组)、40例心胸外科开胸手术和肺部纤维支气管镜检查所取经病理证实的正常肺组织(正常组)作为研究对象。采用免疫组织化学SP法检测两组标本中的Notch1和Bmi-1蛋白表达情况,并分析Notch1、Bmi-1蛋白表达与肺癌患者临床病理特征的关系。结果与结论:肺癌组Notch1、Bmi-1蛋白表达阳性率分别为61%、47%,均显著高于正常组标本(P<0.05);肺癌组Notch1蛋白表达与Bmi-1蛋白表达具有显著的正相关关系(r=0.567,P<0.001)。不同性别、不同病理类型患者的Notch1、Bmi-1蛋白阳性表达率差异无显著性意义(P>0.05);低分化肺癌、发生淋巴结转移、TNM分期Ⅲ-Ⅳ级患者的Notch1、Bmi-1蛋白阳性表达率显著高于高中分化肺癌、无淋巴结转移、TNM分期Ⅰ、Ⅱ级患者(P<0.05)。结果表明Notch1基因和Bmi-1基因可能与肺癌的发生、发展有一定的关系。BACKGROUND： Studies have found that Notch pathway and Bmi-1 gene both have the ability to regulate stem cell self-renew. Functional dysfunction of the both may have a great relationship with tumorigenesis. OBJECTIVE： To investigate the expression and clinical significance of Notch1 and Bmi-1 protein in lung tissue. METHODS： Eighty-seven lung cancer tissue samples（lung cancer group） and forty pathologically confirmed normal lung tissue samples（normal group） were obtained from related surgeries and included as research objects. The protein expression of Notch1 and Bmi-1 in specimens of these two groups was measured by immunohistochemistry SP method. The relationship between Notch1 and Bmi-1 protein expression and clinicopathological features of lung cancer patients was analyzed. RESULTS AND CONCLUSION： The positive rate of Notch1, Bmi-1 protein expression was respectively 61% and 47%, which was significantly higher in the lung cancer group than that in the normal group（P〈0.05）. In the lungcancer group, Notch1 protein expression was significantly positively correlated with Bmi-1 protein expression（r=0.567, P 〈0.001）. There were no significant differences in Notch1 and Bmi-1 protein expression rates between different genders and different pathological types of patients（P〈0.05）. The Notch1 and Bmi-1 protein positive expression rates in poorly-differentiated, TNM stage III-IV lung cancer patients with lymph node metastasis were significantly higher than those in well- and moderately-differentiated, TNM stage I-II lung cancer patients without lymph node metastasis（P〈0.05）. These results demonstrate that Notch1 and Bmi-1 protein may have certain relationship with the occurrence and development of lung cancer.

关 键 词：肿瘤干细胞 肺肿瘤 受体 Notch 组织工程 实验动物 心肺损伤及修复动物模型 NOTCH1基因 BMI-1基因 肺癌组织 

分 类 号：R318[医药卫生—生物医学工程]