头颈部不同部位肿瘤动物模型的建立及生长转移特性比较  

Establishing tumor-bearing animal models at different sites of the head and neck and their growing and metastatic characteristics

作　　者：李文晋[1] 牛金亮[1] 朱莉[1] 王涛[1] 王瑜[1] 余佳丽[1] 

机构地区：[1]山西医科大学第二医院,山西省太原市030001

出　　处：《中国组织工程研究》2016年第5期748-753,共6页Chinese Journal of Tissue Engineering Research

基　　金：山西省卫生厅科技攻关计划项目(20100141);山西省基础研究青年科技研究基金项目(2011021035-4);山西省研究生优秀创新项目(20093066);山西医科大学第二医院博士启动基金(2013-6)~~

摘　　要：背景:建立头颈部肿瘤的动物模型,为进一步研究其发病及转移机制,寻找积极有效的诊断及治疗方案有重要的临床意义。目的:比较头颈部不同部位动物模型肿瘤的生长特性、淋巴结转移及远处转移的特性。方法:VX2肿瘤细胞株复苏培养传代后建立荷瘤种兔,麻醉后从荷瘤处剥离肿瘤,制成肿瘤组织细胞悬液,在兔大腿内侧肌肉处注入瘤细胞悬液,制成传代兔,2周后传代。从传代兔大腿内侧获取肿瘤,制备肿瘤组织悬液,分别在兔耳部、舌部和鼻咽部注入,建立兔头颈部VX2肿瘤模型。结果与结论:3组兔精神、饮食、活动等存在明显差异,耳部移植兔明显好于舌部和鼻咽部。兔耳部、舌部、鼻咽部接种VX2肿瘤细胞悬液,2周后均可成功的建立兔头颈部VX2肿瘤模型,成瘤率耳部最高,为100%(15/15),舌部和鼻咽部成瘤率为93%(14/15);不同部位VX2肿瘤模型经历了肿瘤迅速增长、中心坏死、表面破溃等阶段,生存期为4-6周;3组均出现头颈部淋巴结转移以及肺转移。苏木精伊红染色证实兔VX2肿瘤组织以及转移淋巴结为中至低分化鳞状细胞癌。说明VX2肿瘤组织细胞悬液接种建立的兔头颈部肿瘤模型具有建模周期短,稳定性好、易于重复、移植瘤成功率高、操作简单等特点,兔头颈部不同部位VX2瘤生长各具特点,可根据研究目的选择不同的种植部位。BACKGROUND： To establish a tumor-bearing animal model of the head and neck is of clinical significance for further studying pathogenesis and transfer mechanisms and for actively finding an effective diagnosis and therapeutic regimen. OBJECTIVE： To compare the growth, lymphatic metastasis and distant metastasis characteristics of head and neck tumors in animals. METHODS： VX2 tumor cell lines were resuscitated and passaged to establish tumor-bearing rabbit models. Under anesthesia, the tumor was stripped from the tumor-bearing site to make tumor cell suspension. Then, thecell suspension was injected into the thigh muscle of rabbits and then passaged 2 weeks later. Tumor samples were harvested from the thigh of passage rabbits to make VX2 tumor cell suspension that was injected into the ear, tongue and nasopharynx of rabbits to make a VX2 tumor model of the head and neck in the rabbit. RESULTS AND CONCLUSION： The three groups of rabbits had significant differences in spirit, diet, activity and so on. Rabbits with tumor xenograft of the ear were significantly better than those with tumor xenograft of the tongue and nasopharynx. At 2 weeks after injection of VX2 tumor cell suspension, the VX2 tumor models of the head and neck were made successfully in rabbits, and the tumor formation rate was 100%（15/15） at the ear, 93%（14/15） at the tongue and nasopharynx. VX2 tumors at different sites experienced rapid growth period, central necrosis period, surface ulceration period, and the life cycle was about 4-6 weeks. Lymph node metastasis in head and neck and pulmonary metastasis were found in the all three groups. Hematoxylin-eosin staining showed that VX2 tumor tissues and metastatic lymph nodes were confirmed as moderately to poorly differentiated squamous cell carcinoma. These findings indicate that tumor-bearing rabbit models of the head and neck established by VX2 cell suspension are characterized by short modeling cycle, good stability, easy to repeat, high tumor formation rate and simple operation. VX2

关 键 词：癌 鳞状细胞 肿瘤转移 肿瘤 模型 动物 组织工程 实验动物 细胞损伤与修复模型 头颈部肿瘤 鳞状细胞癌 VX2瘤株 动物模型 兔 淋巴结转移 

分 类 号：R318[医药卫生—生物医学工程]