混合感染猪繁殖与呼吸综合征和圆环病毒2型猪群的猪瘟抗体变化研究  

Study on the Changes of Antibody to Classical Swine Fever in the Swine Herds Co-infected with PRRSV and PCV-2

作　　者：杨苗[1] 李建臻[2] 林华[1] 陈世界[1] 徐刚[2] 

机构地区：[1]四川出入境检验检疫局,四川成都610041 [2]成都农业科技职业学院,四川成都611130

出　　处：《中国动物检疫》2016年第3期5-9,共5页China Animal Health Inspection

基　　金：四川出入境检验检疫局自立科技项目计划(SK201403)

摘　　要：本试验研究旨在探究猪场发生猪繁殖与呼吸综合征(PRRS)与圆环病毒2型(PCV2)混合感染后,猪群的猪瘟抗体变化,运用RT-PCR、实时荧光RT-PCR与PCR方法,对四川某规模化发病猪场进行高致病性PRRS、猪瘟与圆环2型的病原检测。结果显示高致病性PRRS、圆环2型和猪瘟阳性率分别为54%(19/35)、50%(17/34)、0(0/5)。结合临床症状、病猪剖检变化,确诊为PRRSV与PCV-2混合感染。采集PRRS与PCV-2混合感染病猪血清12份为试验组,本场健康猪只(无临床症状、抗原检测阴性)12份为对照组,运用ELISA试验检测猪群猪瘟抗体,结果显示试验组的猪瘟抗体合格率为33%(4/12),显著低于健康对照组的合格率83.3%(10/12)(P<0.05),结果表明PRRS和PCV2混合感染会对猪瘟抗体产生负面影响。To analyze and detect the antibody to CSFV in the swine herds co-infected with PRRSV and PCV-2. RTPCR,real-time fluorescence RT-PCR and PCR methods were used to detect the highly pathogenic PRRSV,CSFV and PCV-2 in the deseased swine farm of Sichuan. The results showed that the positive rates of highly pathogenic PRRSV,PCV-2 and CSFV were 54%（19/35）,50%（17/34）and 0（0/5） respectively. Combined with clinical symptoms and autopsy change,the diagnosis results were co-infection with PRRSV and PCV-2. CSFV antibody was detected by ELISA in the 12 serum samples of experimental group of pigs co-infected with PRRSV and PCV-2 and in the 12 serum samples of control group of healthy pigs（no clinical symptoms,negative antigen detection）. The results showed that CSFV antibody qualified rate was 33%（4/12）in the infected pigs group,significantly lower than that of the healthy control group with the qualified rate of 83.3%（10/12）（（P〈0.05）.It was indicated that there was negative effect on CSFV antibody production with mixed infection of PRRSV and PCV-2.

关 键 词：猪繁殖与呼吸综合征 圆环病毒2型 混合感染 猪瘟抗体 

分 类 号：S851.3[农业科学—预防兽医学]