两种O型口蹄疫ELISA检测试剂盒的对比  

Comparison of Two ELISA Kits for Detection of Type O FMDV Antibody

作　　者：吕晓丽[1] 姚晓韵 何奇松[2] 冯淑萍[2] 马军[1] 覃雨阳 李雪梅[1] 熊毅[2] 颜健华[2] 

机构地区：[1]广西大学动物科学技术学院,广西南宁530005 [2]广西动物疫病预防控制中心,广西南宁530001

出　　处：《中国动物检疫》2016年第3期71-73,共3页China Animal Health Inspection

基　　金：广西科技厅科技攻关项目(桂科攻0779001)

摘　　要：为比较哪种ELISA试剂盒能够更准确、简便、快捷地检测出口蹄疫病毒抗体,本研究使用液相阻断酶联免疫吸附试验(LB-ELISA)试剂盒和固相竞争酶联免疫吸附试验(SPC-ELISA)试剂盒,对2014年广西各市县动物疫病预防控制中心送检的已免疫过口蹄疫疫苗的猪、牛、羊血清共计1121份进行了检测,对比两种试剂盒的特异性、敏感性、阳性检出率及符合率。检测结果表明,两种口蹄疫ELISA试剂盒的阳性检出率不同,LB-ELISA的阳性检出率比SPC-ELISA高2.4%;LB-ELISA试剂盒对血清抗体滴度水平的要求相比SPC-ELISA试剂盒要低,因此LB-ELISA试剂盒更适合于口蹄疫病毒感染的检测。Aimed to compare which of the ELISA kits could detect antibodies against foot-and-mouth disease virus more easily,accurately and quickly,1 121 serums of swine,bovine and sheep immunized foot-and-mouth disease vaccine provided by animal disease prevention and control center of various cities in Guangxi province were tested by LB-ELISA kits and SPC-ELISA kits. The sensitivity,specificity,antibody positive ratio and coincidence rate between the LB-ELISA kits and SPC-ELISA kits were compared. The results showed that the positive rates of two kinds of ELISA kits were different.The positive rate of LB-ELISA was 2.4% higher than that of SPC-ELISA.The requirment of antibody level of LB-ELISA kit was lower than that of SPC-ELISA kit.So the LB-ELISA kits were more suitable for detecting the footand-mouth disease virus infection.

关 键 词：O型口蹄疫 抗体 液相阻断酶联免疫吸附试验 固相竞争酶联免疫吸附试验 比较 

分 类 号：S852.5[农业科学—基础兽医学]