莪术油乳膏对大鼠乳腺增生的作用  

Effects of Curcuma Oil Emulsion on the Hyperplasia of Mammary Glands in Rats

作　　者：李文静[1] 蔡德富[1] 牛英才[1] 李晓明[1] 李成冲[1] 邹宇[1] 洪博[1] 

机构地区：[1]齐齐哈尔医学院药学院,齐齐哈尔161006

出　　处：《医药导报》2016年第3期229-232,共4页Herald of Medicine

基　　金：国家自然科学基金青年基金项目(81403173);黑龙江省卫生厅科研课题项目(2012-327)

摘　　要：目的观察外用莪术油乳膏对乳腺增生模型大鼠的影响,并探讨其作用机制。方法将60只雌性Wistar大鼠随机分为正常对照组、模型对照组、散结乳癖贴膏组和莪术油乳膏大、中、小剂量组,每组10只。采用大鼠后腿内侧肌内注射苯甲酸雌二醇联合黄体酮法复制乳腺增生模型,给予不同剂量药物干预,连续4周。酶联免疫吸附法、苏木精-伊红(HE)染色法和免疫组化法探讨外用莪术油对乳腺增生的作用机制。结果莪术油乳膏对乳腺增生有一定防治作用。与模型对照组比较,莪术油大剂量组大鼠乳房直径显著缩小(P<0.01),体质量恢复正常,血清雌二醇(P<0.01)、促卵泡激素(P<0.05)、泌乳素(P<0.01)均明显降低,孕酮、睾酮、促黄体生成素含量升高(P<0.01),小叶内腺泡数量及直径显著减少。结论莪术油乳膏能显著改善大鼠血清激素水平紊乱,抑制乳腺增生发生。Objective To observe the effect of external use of Curcuma oil on the hyperplasia of mammary glands in rats and explore the mechanism. Methods Sixty female Wistar rats were randomly divided into following groups：normal con-trol group, model control group, Sanjierubi plaster group, low-, medium- and high-dose curcuma oil emulsion groups （ n=10 each）.The models of hyperplasia of mammary glands were established by intramuscular injection of estradiol benzoate and proges-terone into the medial part of the rat hind limb.Different doses of medicines were given for 4 consecutive weeks.Enzyme-linked im-munosorbent assay, HE staining and immunohistochemistry were used to investigate the action mechanism of curcuma oil emulsion against mammary gland hyperplasia. Results Curcuma oil emulsion had preventive and therapeutic effects on the hyperplasia of mammary glands.The diameter of breasts was significantly reduced, the body weight restored, serum estradiol, follicle-stimulating hormone and prolactin levels profoundly decreased, progesterone, testosterone and luteinizing hormone levels markedly increased and the number and diameter of lobular acini obviously reduced in high-dose curcuma oil emulsion group when compared with those in model control group （P〈0.05 or P〈0.01）. Conclusion Curcuma oil emulsion can remarkably improve the disturb-ance of serum hormones and inhibit the occurrence of hyperplasia of mammary glands.

关 键 词：莪术油乳膏 增生 乳腺 病理形态 

分 类 号：R286[医药卫生—中药学] R965[医药卫生—中医学]