葫芦茶乙醇提取物对肝损伤小鼠的保护作用  

Protective Effects of Ethanol Extracts of Tadehagi triquetrum on Acute Liver Injury in Mice

作　　者：唐爱存[1] 陈兆霓[2] 梁韬[2] 卢秋玉[3] 

机构地区：[1]广西中医药大学第一附属医院药学部,南宁530023 [2]广西医科大学药学院药理教研室,南宁530021 [3]广西壮族自治区人民医院药学部,南宁530021

出　　处：《医药导报》2016年第3期242-245,共4页Herald of Medicine

基　　金：广西中医药大学校级科研项目(P2012092)

摘　　要：目的研究葫芦茶乙醇提取物(TTOE)对四氯化碳(CCl4)诱导肝损伤小鼠的保护作用。方法将昆明种小鼠随机分为正常对照组、模型对照组、联苯双酯滴丸组和葫芦茶乙醇提取物小、中、大剂量组,除正常对照组外。其他各组建立化学性肝损伤模型,观察肝脏指数,脾脏指数和胸腺指数,检测小鼠血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、乳酸脱氢酶(LDH)、碱性磷酸酶(ALP)活性,血清清蛋白(Alb)含量以及总抗氧化能力(T-AOC),检测肝组织匀浆超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性,并测定还原型谷胱甘肽(GSH)、丙二醛(MDA)和肝微粒体一氧化氮(NO)及肝微粒体细胞色素P450(Cyt P450)含量。结果与模型对照组比较,葫芦茶乙醇提取物各剂量组肝脏指数、脾脏指数和胸腺指数降低,其中葫芦茶乙醇提取物大剂量组分别为57.13±0.71,32.44±0.24,27.78±0.16,与对照组比较均差异有统计学意义(P<0.01);葫芦茶乙醇提取物各剂量组血清ALT、AST、LDH和ALP活性均显著降低,其中葫芦茶乙醇提取物大剂量组分别为65.59±8.23,141.38±15.52,2 462.4±253.6,172.51±20.64,与对照组比较均差异有统计学意义(P<0.01);葫芦茶乙醇提取物各剂量组血清Alb含量及T-AOC水平升高肝微粒体NO含量和MDA含量降低,肝组织SOD、GSH-PX活性、GSH含量及Cyt P450含量增加(P<0.05或P<0.01)。结论 TTOE对CCl4所致肝损伤小鼠具有保护作用,其机制可能与其抗氧自由基、降低肝微粒体NO含量和抑制脂质过氧化作用有关。Objective To investigate the protective effects of ethanol extracts of Tadehagi triquetrum （ TTOE） on car-bon tetrachloride （ CCl4 ）-induced acute liver injury in mice. Methods Kunming mice were randomly divided into six groups：normal control group （ NC group） ,model control group,bifendate dropping pill group,low-,medium-and high-dose TTOE groups. The liver injury model was established by administration of CCl4 in all the groups except the NC group.The indexes of the liver, spleen and thymus were obtained.The activities of serum ALT,AST,ALP,LDH, albumin and T-AOC were measured.The activi-ties of SOD and GSH-PX and the contents of MDA,NO and GSH and Cyt P450 were also detected in hepatic tissues. Results TTOE at different doses could obviously reduce the indexes of the liver,thymus and spleen,which were （57.13±0.71）,（32.44± 0.24）,and （27.78±0.16）,respectively,in high-dose TTOE group,and there were significant differences between the TTOE groups and model control group （P〈0.01）.The activities of ALT,AST,ALP and LDH were obviously decreased in high-dose TTOE groups,which were （65.59±8.23）,（141.38±15.52）,（2 462.4±253.6）,（172.51±20.64）,respectively,in the TTOE high-dose group （P〈0.01）.The serum levels of Alb and T-AOC were obviously increased,the contents of NO and MDA significantly decreased and the activities of SOD and GSH-PX and the contents of GSH Cyt P450 in liver tissues profoundly increased in TTOE groups when compared with those in model control group （ P〈0.05 or P〈0.01） . Conclusion TTOE could protect against acute hepatic injury induced by CCl4 in mice,which may be associated with the decrease in the activities of liver enzymes,anti-oxide free radical effect,decreased NO content and inhibited lipid peroxidation.

关 键 词：葫芦茶乙醇提取物 损伤 肝 抗氧自由基 脂质过氧化作用 

分 类 号：R286[医药卫生—中药学] R965[医药卫生—中医学]