倒心盾翅藤对肾小管上皮细胞损伤的保护作用  

Preventive Effects of Aspidopterys obcordata on Renal Tubular Epithelial Cells Injury

作　　者：宋美芳[1] 李宜航[1] 李学兰[1] 张忠廉[1] 吕亚娜[1] 李光[1] 

机构地区：[1]中国医学科学院药用植物研究所云南分所云南省西双版纳傣族自治州傣药南药重点实验室,景洪666100

出　　处：《医药导报》2016年第3期249-252,共4页Herald of Medicine

基　　金：中央级公益性科研院所基本科研业务专项(YZYN-14-04,YZYN-15-06)

摘　　要：目的考察傣药倒心盾翅藤不同极性部位在体外对草酸钠致肾小管上皮细胞(HK-2)损伤的保护作用,研究其治疗尿石症的物质基础。方法以草酸钠损伤HK-2细胞模型体外活性筛选为导向,MTT法检测倒心盾翅藤不同极性部位对草酸钠致HK-2细胞损伤的保护作用,初步确定活性部位。结果倒心盾翅藤不同溶剂提取液可不同程度提高草酸钠损伤后细胞活力,0.5和1 mg·mL-1水提液组细胞活力分别上升至86.17%和95.42%;0.5和1 mg·mL-150%醇提液组细胞活力分别上升至93.59%和84.77%;95%醇提液各剂量组细胞活力均上升至81.56%以上,与模型对照组比较,均差异有统计学意义;0.5和1 mg·mL-195%醇提乙酸乙酯部位组细胞活力分别上升至82.53%和91.58%;0.5和1 mg·mL-195%醇提正丁醇部位组细胞活力分别上升至77.24%和87.22%;0.5和1 mg·mL-1水部位组细胞活力分别上升至95.46%和81.36%,与模型对照组比较,均差异有统计学意义,其余部位各剂量组细胞活力与模型对照组比较,差异无统计学意义。结论倒心盾翅藤水提液和醇提液对草酸钠导致的HK-2细胞损伤有明显保护作用,其中乙酸乙酯部位、正丁醇部位以及水部位作用显著,可能是其体外抗结石活性的有效部位。Objective To explore the preventive effects of different extracts from Aspidopterys obcordata on renal tubu-lar epithelial cells injury induced by sodium oxalate in vitro, and initially identify the effective part for treating urolithiasis. Methods The injury model of HK-2 cells induced by sodium oxalate was established to screen the active parts of Aspidopterys obcordata by testing the protective effects of different polarity extracts on HK-2 injury cells through MTT method. Results Dif-ferent extracts from Aspidopterys obcordata improved the activity of HK-2 injury cells, which were elevated to 86.17% and 95.42%by 0. 5 mg-mL-1 and 1 mg-mL-1 aqueous extract, respectively. And the activity reached to 93. 59% and 84. 77% by 0.5 mg-mL-1 and 1 mg-mL-1 50% alcohol extracts, reached above 81.56% by 95% alcohol extracts,all of which showed sig-nificant difference compared with the model group. The HK-2 cells viability were elevated to 82. 53% and 91. 58% by 0.5 mg-mL-1 and 1 mg-mL-1 95% alcohol extracted ethyl acetate parts,and increased to 77.24% and 87.22% by 0.5 mg-mL-1and 1 mg-mL-1 of 95% alcohol isolated n-butanol extracts, approached to 95.46% and 81.36% by 0.5 mg-mL-1 and 1 mg-mL-1 water extracts, all of which showed significant difference compared with the model control group. Conclusion The aqueous extracts and alcohol extracts from Aspidopterys obcordata have obvious preventive effects on HK-2 cells injury, among which the ethyl acetate extracts, n-butanol extracts and water extracts present the remarkable effects, which are supposed to be the active parts for inhibiting calcium oxalate stone formation in vitro.

关 键 词：倒心盾翅藤 草酸钠 HK-2细胞 损伤 上皮细胞 肾小管 提取 不同溶剂 

分 类 号：R286[医药卫生—中药学] R965[医药卫生—中医学]