咽炎糖浆质量控制  

Quality Control of Yanyan Syrup

作　　者：胡北[1] 姚东[1] 王琳琳[1] 史英[1] 史国兵[1] 

机构地区：[1]沈阳军区总医院药剂科,沈阳110016

出　　处：《医药导报》2016年第3期285-288,共4页Herald of Medicine

基　　金：军队医疗机构制剂标准提高科研专项课题(13ZJZ01-3)

摘　　要：目的建立咽炎糖浆的质量标准。方法采用薄层色谱法对方中葛根、玄参进行定性鉴别;采用高效液相色谱法对葛根素进行含量测定:色谱柱为Diamonsil C18(200 mm×4.6 mm,5μm),流动相为甲醇-0.5%醋酸溶液(25∶75),流速为1.0 m L·min^(-1),检测波长为250 nm。结果薄层鉴别斑点清晰、分离较好,阴性对照无干扰;葛根素浓度在3~120μg·mL^(-1)范围内与峰面积呈良好的线性关系(r=0.999 7),平均加样回收率97.44%,RSD=2.07%(n=6)。结论所用定性与定量方法简便、专属性强,结果准确、可靠,所建标准可用于咽炎糖浆的质量控制。Objective To establish the quality standard for Yanyan syrup. Methods Thin layer chromatography （ TLC） was used for the qualitative identification of Puerariae Lobatae Radix and Scrophulariae Radix. High performance liquid chromatography （HPLC） was used to determine the content of puerarin on Diamonsil C18（200 mm×4.6 mm,5μm） column with mobile phase consisting of methanol-0.5% acetic acid （25：75） at a flow rate of 1.0 mL-min-1.The detection wavelength was set at 250 nm. Results TLC spots were clear and well-separated without negative interference.The linear range of puerarin was 3-120μg-mL-1（ r=0.999 7） with an average recovery of 97.44% （ RSD=2.07%,n=6） . Conclusion The method for quality and quantity of Yanyan syrup is simple, specific, accurate and reliable.It can be used for the quality control of Yanyan syrup.

关 键 词：咽炎糖浆 葛根素 质量标准 色谱法 薄层 色谱法 高效液相 

分 类 号：R286[医药卫生—中药学] R927.1[医药卫生—中医学]