化痰活血方对哮喘小鼠IL-17A和IL-13的影响  

Effects of Huatan Huoxue Decoction on IL-17A and IL-13 in asthmatic mice

作　　者：何丽霞[1] 吴赛春[1] 李秀存[1] 凃苑青 曾静[1] 李满萍[1] 杨丽[1] 童晓云[2] 黄丰[3] 

机构地区：[1]暨南大学药学院,广东广州510632 [2]云南中医学院第一附属医院,云南昆明650021 [3]云南中医学院药学院,云南昆明650500

出　　处：《中成药》2016年第3期490-494,共5页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金面上项目(81073129;81560681)

摘　　要：目的研究化痰活血方(川芎、当归、白芍等)对哮喘小鼠支气管肺泡灌洗液(BALF)中白细胞介素IL-17A、IL-13和肺组织血管内皮生长因子-2(VEGFR2)表达的影响。方法小鼠用卵清蛋白(OVA)腹腔注射致敏,并予2%OVA雾化吸入激发的方式建立哮喘小鼠模型,HE染色观察肺组织病理炎症,PAS染色观察肺组织杯状细胞增生情况,酶联免疫法(ELISA)测定BALF中细胞因子IL-17A和IL-13的量,免疫印迹法(Western blot)测定蛋白VEGFR2在肺组织中的表达。结果哮喘组肺部的炎症评分、杯状细胞增生、BALF中IL-17A和IL-13与蛋白VEGFR2的量均明显高于正常组(P<0.05,P<0.01)。与哮喘组比较,化痰活血方组小鼠肺组织病理炎症和杯状细胞增生均减轻,BALF中IL-17A和IL-13的量降低(P<0.05,P<0.01),而肺组织中蛋白VEGFR2的表达无明显变化(P>0.05)。结论化痰活血方可能通过调节IL-17A和IL-13途径来缓解哮喘小鼠症状。AIM To study the effects of Huatan Huoxue Decoction（ Chuanxiong Rhizoma,Angelicae sinensis Radix,Paeoniae Radix alba,etc.） on IL-17 A and IL-13 in bronchoalveolar lavage fluid（ BALF） and the expression of VEGFR2 in lung tissues of asthmatic mice. METHODS The asthmatic model of mice was established by the intraperitoneal injection of OVA followed by atomization inhalation of 2% OVA. The degree of inflammation in lung tissue was evaluated by HE and PAS staining,IL-17 A and IL-13 were detected by ELISA,and the expression of protein VEGFR2 in lung tissue was measured by Western blot. RESULTS Compared with the control group,the score of inflammation,goblet cell hyperplasia,IL-17 A and IL-13 in bronchoalveolar lavage fluid and the expression of protein VEGFR2 in the lung tissue of asthma group were significantly higher（ P〈0. 05,P〈0. 01）.In the Huatan Huoxue Decoction treatment group,the inflammation in lung tissue and goblet cells hyperplasia were alleviated,and the levels of IL-17 A and IL-13 in BALF were decreased（ P〈0. 05,P〈0. 01）. However,the expression of protein VEGFR2 showed no obvious change in lung tissue as compared with that in the asthma group（ P〉0. 05）. CONCLUSION Huatan Huoxue Decoction has certain therapeutic effect on asthmatic mice,which may be through adjusting the pathways of IL-17 A and IL-13.

关 键 词：化痰活血方 哮喘 IL-17A IL-13 支气管肺泡灌洗液(BALF) 卵清蛋白(OVA) 

分 类 号：R285.5[医药卫生—中药学]