UHPLC法同时测定六味香连胶囊中5种成分  

Simultaneous determination of five constituents in Liuwei Xianglian Capsules by UHPLC

作　　者：罗晨煖 罗跃华[1,2] 陈伟康 郑东昆 黄惠明[1] 

机构地区：[1]南昌大学药学院,江西南昌330006 [2]江西省药品检验检测研究院,江西省药品与医疗器械质量工程技术研究中心,江西南昌330029

出　　处：《中成药》2016年第3期556-559,共4页Chinese Traditional Patent Medicine

摘　　要：目的建立超高效液相色谱（UHPLC）法同时测定六味香连胶囊（木香、盐酸小檗碱、枳实等）中柚皮苷、橙皮苷、盐酸小檗碱、和厚朴酚、厚朴酚的含有量。方法该药物70%甲醇提取物的分析采用Agilent XDB-C18色谱柱（4.6 mm×50 mm,1.8μm）;乙腈（A）-0.2%磷酸（B）为流动相,梯度洗脱;体积流量0.7 mL/min;检测波长294 nm;进样体积1μL;柱温40℃。结果柚皮苷、橙皮苷、盐酸小檗碱、和厚朴酚、厚朴酚分别在3.78-189.20μg/mL（r=0.999 5）、1.28-64.00μg/mL（r=0.999 5）、26.78-1339.20μg/mL（r=0.999 7）、1.48-74.00μg/mL（r=0.999 5）、1.33-66.60μg/mL（r=0.999 9）范围内呈良好的线性关系,平均加样回收率（n=9）分别为98.8%、99.0%、99.6%、98.7、100.1%,RSD分别为1.7%、1.6%、1.4%、2.2%、1.6%。结论该方法简便快速,重复性好,可用于六味香连胶囊的质量控制。AIM To establish an ultra high performance liquid chromatography（ UHPLC） method for the simultaneous determination of naringin,hesperidins,berberine hydrochloride,honokiol and magnolia in Liuwei Xianglian Capsules（ Aucklandia lappa,berberine hydrochloride,Fructus aurantii immaturus,etc.）. METHODS The analysis of 70% methanolic extract of this drug was performed on Agilent XDB-C18column（ 4. 6 mm × 50 mm,1. 8 μm）,mobile phase was acetonitrile（ A）-0. 2% formic acid（ B） in a gradient elution manner,detection wavelength was set at 294 nm,injection volume was 1 μL,and column temperature was maintained at 40 ℃. RESULTS Naringin,hesperidins,berberine hydrochloride,honokiol and magnolia displayed good linear relationships within the ranges of 3. 78- 189. 20 μg / mL（ r = 0. 999 5）,1. 28- 64. 00 μg / mL（ r = 0. 999 5）,26. 78-1 339. 20 μg / mL（ r = 0. 999 7）,1. 48- 74. 00 μg / mL（ r = 0. 999 5） and 1. 33- 66. 60 μg / mL（ r =0. 999 9）,and their average recoveries（ n = 9） were 98. 8%,99. 0%,99. 6%,98. 7% and 100. 1% with the RSDs of 1. 7%,1. 6%,1. 4%,2. 2% and 1. 6%,respectively. CONCLUSION This method is simple,rapid and reproducible,which can be applied to the quality control of Liuwei Xianglian Capsules.

关 键 词：六味香连胶囊 柚皮苷 橙皮苷 盐酸小檗碱 和厚朴酚 厚朴酚 UHPLC 

分 类 号：R927.2[医药卫生—药学]