HIV/HCV共感染者T淋巴细胞亚群CD_(28)、CD_(38)分子表达特征的分析  

The Expression Characteristics of CD_(28), CD_(38) Molecular on CD_8^+ T Lymphocytes of HIV/HCV Co-Infected Patients

作　　者：杨国强[1] 

机构地区：[1]郑州人民医院检验科,河南郑州450000

出　　处：《中国医药指南》2016年第7期5-6,8,共3页Guide of China Medicine

摘　　要：目的通过观察HIV/HCV共感染者外周血T淋巴细胞亚群CD_(28)、CD_(38)分子的表达规律,探讨其与疾病进展的相关性。方法本研究选择本院2012年8月至2014年10月门诊HIV感染者40例、HIV/HCV共感染患者40例及体检中心2014年9~10月体检健康人群20例,采用流式细胞术检测HIV/HCV共感染患者(40例)、HIV感染者(40例)和健康人(20例)外周血CD_8^+T淋巴细胞表面CD_(28)、CD_(38)分子的表达,分析HIV/HCV共感染者CD_(28)、CD_(38)分子在T淋巴细胞亚群的表达特征;按照CD4^+T数值对HIV/HCV共感染者进行分组,分析不同CD4^+T水平CD_8^+CD_(28)^+、CD_8^+CD_(38)^+的表达差异,探讨CD_8^+T淋巴细胞CD_(28)、CD_(38)分子与疾病进展的相关性。结果与健康人群及HIV感染者比较,HIV/HCV共感染者CD_8^+CD_(38)^+T细胞的绝对计数与百分比明显升高,CD_8^+CD_(28)^+T细胞百分比明显降低;随着CD4^+T水平的降低,CD_8^+CD_(28)^+T有逐渐降低趋势,CD_8^+CD_(38)^+T有逐渐升高趋势。结论 HIV/HCV共感染者CD_8^+T淋巴细胞表面CD_(28)分子表达降低,CD_(38)分子表达升高,且这种变化与患者CD4^+T水平密切相关,可作为判断疾病进展的重要指标。Objective To study the expression of CD28, CD38 molecular on CD8^＋ T cell of peripheral blood of HIV/HCV co-infected patients, and explore its correlation with disease progression. Methods 40 cases of HIV infection and 40 cases of HIV/HCV coinfection come from Outpatient service of our hospital from October 2012 to August 2012, while 20 cases of healthy people were chosen from medical examination center from September 2014 to October check-up 20, the CD28, CD38 molecule expression on CD8^＋ T lymphocytes of 40 HIV/HCV co-infected patients, 40 HIV patients and 20 healthy people were detected by flow cytometry instrument, analyzed the expression characteristics of CD28, CD38 molecular on CD8^＋ T lymphocytes of HIV/HCV co-infected patients; grouped the HIV/HCV co-infected patients according to the CD4^＋ T value, through comparing the different expression of CD8^＋CD28^＋, CD8^＋CD38^＋among different CD4^＋ T groups, discuss the correlation between CD28, CD38 molecules on CD8^＋T lymphocytes and disease progression. Results Compared with healthy people and HIV patients, the percentage of CD8^＋CD28^＋ T cells of HIV/HCV coinfected patients reduced significantly, while the CD8^＋CD38^＋ T cell absolute counting and percentage increased significantly; followed the the reduction of the CD4^＋ T,the CD8^＋CD28^＋ T reduced gradually,while the CD8^＋CD38^＋ T has a trend of increasing gradually. Conclusion The CD8^＋CD28^＋ in peripheral blood of HIV/HCV co-infected patients reduces gradually, while CD8^＋CD38^＋ increased gradually, both of which are closely associated with disease progression, and they can be used as judgement index of disease progression.

关 键 词：HIV/HCV T淋巴亚群 CD8^+CD28^+ CD8^+CD38^+ 疾病进展 

分 类 号：R512.63[医药卫生—内科学] R512.91[医药卫生—临床医学]