miR-29a促进小鼠子宫内膜的蜕膜化  

Mir-29a Promotes Endometrial Decidualization in Mice

作　　者：张鹏[1] 范立青[1,2] 

机构地区：[1]中南大学生殖与干细胞工程研究所 [2]中信湘雅生殖与遗传专科医院,湖南长沙410078

出　　处：《中山大学学报（医学科学版）》2016年第1期1-8,共8页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：国家自然科学基金(30760071)

摘　　要：【目的】探讨微小RNA29a（mi R-29a）在小鼠子宫内膜基质细胞（ESC）蜕膜化中的作用。【方法】收集孕第1~9天（D1~D9）的小鼠子宫,通过实时定量PCR（q RT-PCR）的方法检测小鼠子宫组织中mi R-29a的表达;构建去除卵巢及其甾体激素的处理模型,观察甾体激素对mi R-29a表达的影响。采用雌二醇（E2）和孕酮（P4）对分离培养的小鼠子宫内膜基质细胞进行蜕膜化处理,并通过瞬时转染的方法下调mi R-29a的表达,分别收集蜕膜化处理不同时间及干扰前后的细胞,采用q RT-PCR检测催乳素相关蛋白（d PRP）m RNA的表达,Western blot法检测细胞周期蛋白D3（cyclin D3）、孕酮受体（PR）蛋白的表达。【结果】在小鼠妊娠第1~5天,子宫组织中的mi R-29a表达量较低,随着蜕膜化的进程（妊娠第6~9天）,mi R-29a的表达量均显著性增加（与妊娠D1相比）;在去除卵巢及其甾体激素的处理模型中,一旦给予雌孕激素刺激,mi R-29a的表达量显著增加;在小鼠子宫内膜基质细胞体外诱导蜕膜化过程中,q RT-PCR结果显示d PRP m RNA的表达以及mi R-29a的表达随着蜕膜化时间的延长逐渐升高（与24 h相比）,Western blot法显示蜕膜化标志分子cyclin D3、PR的表达也随着蜕膜化时间的延长逐渐升高;转染下调mi R-29a后,蜕膜化标志分子及mi R-29a的表达也相应下降。【结论】妊娠早期mi R-29a表达于母胎界面,且受子宫内膜蜕膜化的影响,说明mi R-29a具有促进子宫内膜蜕膜化的作用。【Objective】 To explore the effect of micro RNA-29a（mi R-29a） in mouse endometrial stromal cells（ESC） during the decidua process. 【Methods】 To collect uterus of mouse, which were pregnant for 1-9 d, detecting the mi R-29 a expression in uterine tissue of mice by real-time quantitative PCR（q RT-PCR）. The effects of steroid hormones on the expression of mi R-29 a by constructing a mouse model of ovariectomized and processing of sex steroid hormones were observed. Use of estradiol（E2） and progesterone（P4） on decidual mouse endometrial stromal cells in vitro processing, and reduced mi R-29 a expression with the method of transient transfection.Cells in different time of decidualization and interference were collected respectively. The level of prolactin related proteins（d PRP）m RNA was detected by q RT-PCR, and the expression of decidualization marker molecule cyclin D3 and progesterone receptor（PR） was detected by Western blot. 【Results】 The expression of mi R-29 a was relatively low in the uterus of pregnant mice after 1-5 d. With the progress of deciduas（pregnant after 6-9 d）, the level of mi R-29 a significantly increased（compared with that of pregnant mice after 1d）.In the processing model of ovary removal and steroid hormones treatment, the expression of mi R-29 a increased significantly after given estrogen and progesterone stimulatio; q RT-PCR and Western blot showed that the level of d PRP m RNA, mi R-29 a, cyclin D3, PR increased with prolonging of decidualization time; the expression of decidual marker molecules and mi R-29 a also decreased after downregulation of mi R-29 a by transfection. 【Conclusion】 mi R-29 a expresses on the maternal fetal interface in early pregnancy, this is affected by decidua. So indicates that mi R-29 a can promote the progress of endometrial decidualization.

关 键 词：miR-29a 基质细胞 蜕膜化 孕酮 雌二醇 

分 类 号：R392-33[医药卫生—免疫学] R711.51[医药卫生—基础医学]