辽宁碱蓬SlCMO基因盐胁迫表达分析及盐诱导启动子鉴定  

Analysis of Expression Pattern and Inducible Promoter of SlCMO Gene in Suaeda liaotungensis Under Salt Stress

作　　者：佟少明[1] 尹辉[1] 夏冰[1] 李秋莉[1] 

机构地区：[1]辽宁师范大学生命科学学院/辽宁省植物生物技术重点实验室,辽宁大连116081

出　　处：《中国生物化学与分子生物学报》2016年第3期332-338,共7页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家自然科学基金项目(No.30871389)资助~~

摘　　要：胆碱单加氧酶(choline monooxygenase,CMO)是合成甜菜碱的关键酶,甜菜碱在植物抵抗渗透胁迫中起着重要的作用。本研究室前期克隆了盐生植物辽宁碱蓬CMO(Suaeda liaotungensis CMO)基因及启动子。本研究对Sl CMO基因在盐胁迫下的表达及盐诱导启动子进行分析。q RTPCR分析Sl CMO基因在辽宁碱蓬不同器官及盐胁迫下的表达,结果表明,Sl CMO基因在根、茎、叶中均有表达,其中茎、叶中的表达量较高,Sl CMO基因在根、茎、叶中的表达均受盐胁迫诱导。5'端缺失分析Sl CMO启动子的盐诱导区段,结果表明,p C5(-267^+128 bp)是Sl CMO启动子的盐诱导功能区段,推测p C5调控Sl CMO基因的盐诱导表达。本研究为Sl CMO基因表达调控研究奠定基础,也为植物抗盐基因工程提供可用的启动子。Glycine betaine is an osmoticum,playing an important role in plant resistance to osmotic stress. Choline monooxygenase（ CMO） is the key enzyme in glycine betaine synthesis. In this study,we analyzed the expression of Sl CMO in S. liaotungensis and identified the salt-inducible region of its promoter. The expression of S. liaotungensis CMO（ Sl CMO） was higher in stems and leaves than in roots as from q RT-PCR following salt-induction in S. liaotungensis. A 5＇ deletion of the Sl CMO promoter indicated that p C5（- 267 to ＋ 128 bp） was responsible for the salt-inducible response. These results suggested that the expression of Sl CMO was salt-induced and that p C5 regulated salt-inducible Sl CMO expression. This information can be useful in subsequent engineering plant promoters for salt-resistance.

关 键 词：辽宁碱蓬 辽宁碱蓬胆碱单加氧酶(SlCMO) QRT-PCR 启动子 盐胁迫 

分 类 号：Q71[生物学—分子生物学]