耐力运动通过抑制HIF-1α/iNOS预防高脂饮食小鼠脂肪组织炎症  

Endurance Exercise Preventing Adipose Tissue Inflammation by Inhibiting HIF-1α/iNOS

作　　者：邹华刚[1] 陈香仙[1] 

机构地区：[1]安徽师范大学体育学院,安徽芜湖241000

出　　处：《中国体育科技》2016年第2期85-89,104,共6页China Sport Science and Technology

摘　　要：目的:研究耐力运动对高脂饮食小鼠脂肪组织HIF-1α/iNOS信号的影响,探讨运动预防脂肪组织炎症的分子机制。方法:8周龄C57BL/6小鼠40只随机分为正常饮食对照组(NC)、正常饮食运动组(NE)、高脂饮食对照组(HC)、高脂饮食运动组(HE),运动组进行8周跑台运动(13 m/min)。ELISA法测定血清TNF-α、IL-6,RT-PCR法测定基因表达,Western Blot法测定蛋白表达。结果:与NC组相比,HC组小鼠血清TNF-α、IL-6浓度显著升高(P<0.05),脂肪组织SVCs TNF-α、IL-6、IL-1β、HIF-1α、PDK1、LDH-A、GLUT1、iNOS mRNA表达均显著升高(P<0.05),HIF-1α和iNOS蛋白表达显著升高(P<0.05);与NC组相比,NE组小鼠SVCs HIF-1αmRNA和蛋白表达显著下降(P<0.05),其余参数无显著差异;与HC组相比,HE组血清TNF-α、IL-6显著下降(P<0.05),脂肪组织SVCs TNF-α、IL-6、IL-1β、HIF-1α、PDK1、LDH-A、GLUT1、iNOS mRNA表达显著下降,HIF-1α、iNOS蛋白表达显著下降(P<0.05)。结论:8周耐力跑台运动可有效预防高脂饮食小鼠脂肪组织慢性炎症,其机制可能与运动抑制脂肪组织巨噬细胞HIF-1α/iNOS信号轴有关。Objective：The effects of endurance exercise on HIF-1α/iNOS signaling in adipose tissue of high fat diet feeding mice were investigated to explore the molecular mechanisms of exercise-induced prevention from adipose tissue inflammation induced by high fat diet.Methods：40 8-week old C57BL/6 mice were randomly divided into 4groups,namely normal diet control（NC）,normal diet exercise（NE）,high fat diet control（HC）and high fat diet exercise（HE）with each group 10 mice.Exercise group were subjected to 8 week treadmill running program at a speed of 13 m/min.ELISA assay was used to measure serum TNF-αand IL-6.RT-PCR was used to determine gene expression while Western Blot was used to determine protein expression.Results：Compared to NC group,serum TNF-α,IL-6and TNF-α,IL-6,IL-1β,HIF-α,PDK1,GLUT1,LDHA,iNOS mRNA expression as well as HIF-1αand iNOS protein expression in adipose tissue SVCs of HC group were significantly elevated（P〈0.05）.Campared with NC group,HIF-1α mRNA and protein expression in adipose tissue SVCs of NE group were reduced markedly（P〈0.05）while other indicators changed without significance.Compared with HC group,serum TNF-α,IL-6 and TNF-α,IL-6,IL-1β,HIF-α,PDK1,GLUT1,LDH-A,iNOS mRNA expression as well as HIF-1αand iNOS protein expression in adipose tissue SVCs of HE group were significantly reduced（P〈0.05）.Conclusion：8week endurance treadmill running efficiently prevent chronic inflammation of high fat diet feeding mice possibly by inhibiting HIF-1α/iNOS signaling pathway.

关 键 词：耐力运动 脂肪组织 低氧 慢性炎症 胰岛素抵抗 鼠 动物实验 

分 类 号：G804.5[文化科学—运动人体科学]